Abstract
A novel adoptive transfer system was used to track the fate of naive Salmonella-specific CD4 T cells in vivo. These cells showed signs of activation in the Peyer's patches as early as 3 hr after oral infection. The activated CD4 T cells then produced IL-2 and proliferated in the T cell areas of these tissues before migrating into the B cell-rich follicles. In contrast, Salmonella-specific CD4 T cells were not activated in the spleen and very few of these cells migrated to the liver, despite the presence of bacteria in both organs. These results show that the T cell response to pathogenic Salmonella infection is localized to the gut-associated lymphoid tissue and does not extend efficiently to the major sites of late infection.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 365-377 |
| Number of pages | 13 |
| Journal | Immunity |
| Volume | 16 |
| Issue number | 3 |
| DOIs | |
| State | Published - 2002 |
Bibliographical note
Funding Information:We thank Jennifer Walter for expert technical assistance, Drs. Brad Cookson and Damo Xu for providing bacterial strains, Micki Dyer and Sandra Horne from the University of Minnesota Cancer Center ES Cell/SPF Animal Facility for production of transgenic mice, and Gerald Sedgewick for image analysis expertise. This work was supported by a fellowship from the Irvington Institute for Immunological Research (to S.J.M.) and by grants from the National Institutes of Health (AI27998, AI35296, and AI39614 to M.K.J.).