Transcriptional control of the isoeugenol monooxygenase of Pseudomonas nitroreducens Jin1 in Escherichia coli

Ji Young Ryu, Jiyoung Seo, Joong Hoon Ahn, Michael J. Sadowsky, Hor Gil Hur

Research output: Contribution to journalArticlepeer-review

7 Scopus citations


Vanillin is one of the most valuable compounds in the flavoring and fragrance industries, and many attempts to produce natural vanillin have been made in recent years. Isoeugenol monooxygenase (Iem) converts the phenylpropanoid compound isoeugenol to vanillin. In Pseudomonas nitroreducens Jin1, the positive regulatory protein IemR is divergently expressed from Iem, and the promoter region is located between the genes. In this study, we investigated the transcriptional regulation of iem in Escherichia coli. We focused on inducers and regulatory protein IemR. Transcription of iem was found to be dependent on the amounts of isoeugenol and IemR. Isoeugenol was found to be the best inducer of iem, followed by trans-anethole, which induced iem to 58% of the transcription level observed for isoeugenol. Overproduction of IemR in E. coli significantly increased the transcription of iem, up to 96-fold, even in the absence of isoeugenol, as compared to basally expressed IemR. Results of this study indicate that the transcription of iem iss dependent on the type of inducers and on IemR. They should contribute to the development of bioengineering strategies for increased production of vanillin through high-level expression of the isoeugenol monooxygenase gene in microorganisms.

Original languageEnglish (US)
Pages (from-to)1891-1896
Number of pages6
JournalBioscience, Biotechnology and Biochemistry
Issue number10
StatePublished - 2012

Bibliographical note

Funding Information:
This work was supported by a National Research Foundation of Korea (NRF: 2011-0029860) grant.


  • Inducers
  • Isoeugenol monooxygenase
  • Regulator
  • Transcription
  • Vanillin


Dive into the research topics of 'Transcriptional control of the isoeugenol monooxygenase of Pseudomonas nitroreducens Jin1 in Escherichia coli'. Together they form a unique fingerprint.

Cite this