Transcriptional organization of the Escherichia coli pilus adhesin K99

Olivia J. Inoue, John H. Lee, Richard E. Isaacson

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

The production of the Escherichia coli pilus adhesin K99 requires the expression of eight unique proteins: FanA‐H. The transcriptional organization of the K99 operon was investigated by Northern blot analysis. Four RNAs of 0.54, 1.4, 2.5 and 3.5kb were detected. When a fanC probe was used all four RNAs were detected while the use of fanD, fanF and fanG probes detected two RNAs each. Using several deletion and TnphoA insertion mutants it was concluded that there were seven unique K99‐specific transcripts, several of which were of the same approximate sizes (1.4 and 2.5 kb). It also was concluded that K99 was comprised of at least three complementation groups, two of which were regulated by catabolite repression.

Original languageEnglish (US)
Pages (from-to)607-613
Number of pages7
JournalMolecular Microbiology
Volume10
Issue number3
DOIs
StatePublished - Nov 1993

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