Transcriptional profiling and molecular characterization of the yccT mutant link: A novel STY1099 protein with the peroxide stress response and cell division of Salmonella enterica serovar enteritidis

Sinisa Vidovic, Xiaoying Liu, Ran An, Kristelle M. Mendoza, Juan E. Abrahante, Anup K. Johny, Kent M. Reed

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Uncharacterized protein STY1099, encoded by the yccT gene, was previously identified as the most altered (i.e., upregulated) protein among the ZnO nanoparticle (NP) stimulon of Salmonella enterica serovar Enteritidis. Here we combined various stress response-related assays with functional genetics, global transcriptomic and proteomic analyses to characterize the yccT gene and its STY1099 product. Exposure of S. enterica Enteritidis to H2O2 (i.e., hydrogen peroxide) resulted in a significant (p < 0.0001) upregulation of the yccT gene, whereas exposure to paraquat (i.e., superoxide) did not alter the expression of the yccT gene. The ∆yccT mutant of S. enterica Enteritidis exposed to 0.75 mM H2O2, showed significantly reduced (p < 0.05) viability compared to the wild type strain. Further, comparative transcriptome analyses supported by Co-immunoprecipitation (Co-IP) assay revealed that STY1099 protein plays a role in redox homeostasis during the peroxide stress assault via involvement in the processes of respiratory nitrate reductase, oxidoreductase activities, cellular uptake and stress response. In addition, we found that the STY1099 protein has the monopolar subcellular location and that it interacts with key cell division proteins, MinD, and FtsH, as well as with a rod shape-determining protein MerB.

Original languageEnglish (US)
Article number86
JournalBiology
Volume8
Issue number4
DOIs
StatePublished - Dec 2019

Bibliographical note

Funding Information:
Acknowledgments: The authors gratefully acknowledge the technical support from the University Imaging Centers at the University of Minnesota, Jim Imlay, Sergei Korshunov and Daniela Vidovic. We also thank Misara Omar and Sahar Alshalchi for their assistance. This project was supported by the Signature Program AES GAR grant. The present study was also supported, in part, by resources provided by the Minnesota Supercomputing Institute.

Funding Information:
Funding: This research was funded by the United States Department of Agriculture, National Institute of Food and Agriculture, Hatch Capacity Grant # 592 and Grant-in-Aid of Research, Artistry and Scholarship, University of Minnesota, USA to S.V.

Publisher Copyright:
© 2019 by the authors. Licensee MDPI, Basel, Switzerland.

Keywords

  • Cell division
  • Nitrate reductase
  • Peroxide stress response
  • STY1099 protein
  • Salmonella enterica serovar Enteritidis

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