TY - JOUR
T1 - Transcriptional regulation of mouse δ-opioid receptor gene
T2 - Role of Ets-1 in the transcriptional activation of mouse δ-opioid receptor gene
AU - Sun, Ping
AU - Loh, Horace H
PY - 2001/11/30
Y1 - 2001/11/30
N2 - Previously, we identified a minimum core promoter of the mouse δ-opioid receptor (DOR) gene. The DOR promoter contains an E-box that binds upstream stimulatory factor and is crucial for the DOR promoter activity in NS20Y cells, a mouse neuronal cell line that constitutively expresses DOR. In the present study, we further analyzed the DOR promoter in NS20Y cells and have demonstrated that transcription factor Ets-1 binds to an Ets-1-binding site overlapping the E-box and trans-activates the DOR promoter by synergizing with upstream stimulatory factor in specific DNA binding. In addition, the Ets-1 DNA-binding domain is sufficient to play the functional role of Ets-1 in trans-activating the DOR promoter. Furthermore, through in vivo cross-linking assays and Northern blot analyses, we have demonstrated that Ets-1 binds to the DOR promoter in the neonatal mouse brain and that overexpressed Ets-1 can significantly enhance the expression of DOR mRNA in primary neonatal mouse neuronal cells. Collectively, our data suggest that Ets-1 functions as a trans-activator of the DOR promoter in the neonatal mouse brain and thus may contribute to the development of the mouse brain DOR system.
AB - Previously, we identified a minimum core promoter of the mouse δ-opioid receptor (DOR) gene. The DOR promoter contains an E-box that binds upstream stimulatory factor and is crucial for the DOR promoter activity in NS20Y cells, a mouse neuronal cell line that constitutively expresses DOR. In the present study, we further analyzed the DOR promoter in NS20Y cells and have demonstrated that transcription factor Ets-1 binds to an Ets-1-binding site overlapping the E-box and trans-activates the DOR promoter by synergizing with upstream stimulatory factor in specific DNA binding. In addition, the Ets-1 DNA-binding domain is sufficient to play the functional role of Ets-1 in trans-activating the DOR promoter. Furthermore, through in vivo cross-linking assays and Northern blot analyses, we have demonstrated that Ets-1 binds to the DOR promoter in the neonatal mouse brain and that overexpressed Ets-1 can significantly enhance the expression of DOR mRNA in primary neonatal mouse neuronal cells. Collectively, our data suggest that Ets-1 functions as a trans-activator of the DOR promoter in the neonatal mouse brain and thus may contribute to the development of the mouse brain DOR system.
UR - http://www.scopus.com/inward/record.url?scp=0035976956&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0035976956&partnerID=8YFLogxK
U2 - 10.1074/jbc.M104793200
DO - 10.1074/jbc.M104793200
M3 - Article
C2 - 11583993
AN - SCOPUS:0035976956
SN - 0021-9258
VL - 276
SP - 45462
EP - 45469
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 48
ER -