Transcriptional regulatory mechanisms that govern embryonic stem cell fate

Satyabrata Das, Dana Levasseur

Research output: Chapter in Book/Report/Conference proceedingChapter

10 Scopus citations

Abstract

Embryonic stem cells (ESCs) are defined by their simultaneous capacity for limitless self-renewal and the ability to specify cells borne of all germ layers. The regulation of ESC pluripotency is governed by a set of core transcription factors that regulate transcription by interfacing with nuclear proteins that include the RNA polymerase II core transcriptional machinery, histone modification enzymes, and chromatin remodeling protein complexes. The growing adoption of systems biological approaches used in stem cell biology over last few years has contributed significantly to our understanding of pluripotency. Multilayered approaches coupling transcriptome profiling and proteomics (Nanog-, Oct4-, and Sox2-centered protein interaction networks or "interactomes") with transcription factor chromatin occupancy and epigenetic footprint measurements have enabled a more comprehensive understanding of ESC pluripotency and selfrenewal. Together with the genetic and biochemical characterization of promising pluripotency modifying proteins, these systems biological approaches will continue to clarify the molecular underpinnings of the ESC state. This will most certainly contribute to the improvement of current methodologies for the derivation of pluripotent cells from adult tissues.

Original languageEnglish (US)
Title of host publicationEmbryonic Stem Cell Immunobiology
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Pages191-203
Number of pages13
ISBN (Print)9781627034777
DOIs
StatePublished - Jan 1 2013
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume1029
ISSN (Print)1064-3745

Keywords

  • Chromatin immunoprecipitation
  • Interactome
  • Mediator
  • Micro RNA
  • Pluripotency
  • Proteomics
  • RNA polymerase II
  • Self-renewal

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