Transcriptome analysis of heat stress response genes in potato leaves

Ruimin Tang, Sanjay K. Gupta, Suyan Niu, Xiu Qing Li, Qing Yang, Guanshui Chen, Wenjiao Zhu, Muhammad Haroon

Research output: Contribution to journalArticlepeer-review

Abstract

Heat stress has a severe impact on potato growth and tuberization process, always resulting in the decrease of tuber yield and quality. Therefore, it is of great significance for potato breeding to illuminate the mechanism of heat stress on potato and explore heat resistant genes. In this study, two cDNA libraries from normal potato leaves (20 °C day/18 °C night) and potato leaves with 3 days of heat treatment (35 °C day/28 °C night) were constructed respectively. Totally, 1420 differentially expressed genes (DEGs) were identified. The expression patterns of 12 randomly selected genes detected using droplet digital PCR agreed with the sequencing data. Gene ontology analysis showed that these DEGs were clustered into 49 different GO types, reflecting the functional diversity of the heat stress response genes. The results of KEGG pathway enrichment showed the potential biological pathways in which the DEGs were involved, indicating that these pathways may be involved in heat tolerance regulation. Most potato heat transcription factors (StHsfs) and heat shock proteins (StHsps) were not expressed efficiently based on expression profile of these DEGs. StHsp26-CP and StHsp70 were markedly increased after 3 days of heat treatment. These data will be useful for further understanding the molecular mechanisms of potato plant tolerance to heat stress and provide a basis for breeding heat-tolerance varieties.

Original languageEnglish (US)
Pages (from-to)4311-4321
Number of pages11
JournalMolecular Biology Reports
Volume47
Issue number6
DOIs
StatePublished - Jun 1 2020

Bibliographical note

Funding Information:
This research was supported by the funds from the Natural Science Foundation of Jiangsu Province (BK20180519) and the Priority Academic Program Development of Jiangsu Higher Education Institutions: Modern Horticultural Science (PAPD).

Publisher Copyright:
© 2020, Springer Nature B.V.

Keywords

  • Differentially expressed genes
  • Droplet digital PCR
  • Heat stress
  • Potato
  • Transcriptome sequencing

PubMed: MeSH publication types

  • Journal Article

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