TY - CHAP
T1 - Transcriptomic analysis of staphylococcus aureus using microarray and advanced next-generation RNA-seq Technologies
AU - Lei, Ting
AU - Becker, Aaron
AU - Ji, Yinduo
PY - 2014
Y1 - 2014
N2 - The transcriptome has shown tremendous potential for the comprehensive investigation of gene expression profiles and transcriptional levels in comparative biology, the identification of regulatory mechanism of transcriptional regulators, and the evaluation of target gene for developing new chemotherapeutic agents, vaccine, and diagnostic methods. The traditional microarray and advanced next-generation RNA sequencing technologies (RNA-seq) provide powerful and effective tools for the determination of the transcriptome of bacterial cells. In this chapter, we provide a detailed protocol for scientists who want to investigate gene expression profiles by performing microarray and/or RNA-seq analysis, including different RNA purification methods, mRNA enrichment, decontamination, cDNA synthesis, fragmentation, biotin labeling for hybridization using Affymetrix Staphylococcus aureus chips, quantitative real-time reverse transcription PCR, and RNA-seq data analysis.
AB - The transcriptome has shown tremendous potential for the comprehensive investigation of gene expression profiles and transcriptional levels in comparative biology, the identification of regulatory mechanism of transcriptional regulators, and the evaluation of target gene for developing new chemotherapeutic agents, vaccine, and diagnostic methods. The traditional microarray and advanced next-generation RNA sequencing technologies (RNA-seq) provide powerful and effective tools for the determination of the transcriptome of bacterial cells. In this chapter, we provide a detailed protocol for scientists who want to investigate gene expression profiles by performing microarray and/or RNA-seq analysis, including different RNA purification methods, mRNA enrichment, decontamination, cDNA synthesis, fragmentation, biotin labeling for hybridization using Affymetrix Staphylococcus aureus chips, quantitative real-time reverse transcription PCR, and RNA-seq data analysis.
KW - Advanced next-generation RNA sequencing technologies (RNA-Seq)
KW - Gene expression
KW - Microarray analysis
KW - Real-time reverse transcription (RT) polymerase chain reaction (PCR)
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U2 - 10.1007/978-1-62703-664-1_13
DO - 10.1007/978-1-62703-664-1_13
M3 - Chapter
C2 - 24085699
AN - SCOPUS:84934438654
SN - 9781627036634
T3 - Methods in Molecular Biology
SP - 213
EP - 229
BT - Methicillin-Resistant Staphylococcus Aureus (MRSA) Protocols
PB - Humana Press Inc.
ER -