Transforming growth factor‐β1 binds to immobilized fibronectin

Daniel L. Mooradian, Roger C. Lucas, James A. Weatherbee, Leo T. Furcht

Research output: Contribution to journalArticlepeer-review

68 Scopus citations

Abstract

We have characterized the interaction of homodimeric porcine transforming growth factor‐β1 (TGF‐β1) with affinity‐purified human plasma fibronectin. Using a solid‐phase binding assay, we have demonstrated that TGF‐β1 binds to fibronectin immobilized on Immunlon ITM microtiter plates. TGF‐β1 binding increased with time, reaching a plateau after 4–6 h, and was dependent upon the concentration of both labeled TGF‐β1 and immobilized fibronectin present. The binding of radiolabeled TGF‐β1 to fibronectin was saturable and was reduced 75% in the presence of a 100‐fold excess of unlabeled TGF‐β1. TGF‐β1 bound to fibronectin with an association rate constant (Ka) of 2.96 × 103 M−1 s−1 and did not readily dissociate under various conditions. The binding of TGF‐β1 to fibronection was insensitive to variations in ionic strength over a range of 0.1–1.0 M NaCl and was relatively insensitive to divalent cation concentration in the range of 0.1–10.0 mM as well. These data suggest that the binding of TGF‐β1 to fibronectin may not be dependent upon the interaction of charged amino acids within these two molecules. However, the binding of TGF‐β1 to fibronectin was strongly pH‐dependent and binding decreased dramatically below pH 4.0 and above pH 10.0, suggesting that charged amino acids may influence TGF‐β1/fibronectin interactions. The association of TGF‐β1 with immobilized fibronectin or other extracellular matrix components and subsequent dissociation under acidic conditions or by an as‐yet‐unidentified mechanism may play a role in the distribution and/or activity of this potent growth regulator at sites of tissue injury and inflammation in vivo.

Original languageEnglish (US)
Pages (from-to)189-200
Number of pages12
JournalJournal of Cellular Biochemistry
Volume41
Issue number4
DOIs
StatePublished - Dec 1989

Keywords

  • cell differentiation
  • cell growth
  • extracellular matrix
  • fibronectin
  • growth factor binding

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