Abstract
Transforming growth factor-β1 (TGF-β1) plays a central role in the response of the brain to different types of injury. Increased TGF-β1 has been found in the central nervous system of patients with acute or chronic disorders such as stroke or Alzheimer disease. To further define the molecular targets of TGF-β1 in cerebral tissues, a selection of high-density cDNA arrays was used to characterize the mRNA expression profile of 7,000 genes in transgenic mice overexpressing TGF-β1 from astrocytes as compared with the wild-type line. Selected findings were further evaluated by reverse transcription-polymerase chain reactions from independent transgenic and wild-type mice. Furthermore, the expression pattern of seven selected genes such as Delta-1, CRADD, PRSC-1, PAI-1, Apo-I/Fas, CTS-B, and TβR-II were confirmed in either cultured cortical neurons or astrocytes following TGF-β1 treatment. The authors' observations enlarge the repertoire of known TGF-β1-modulated genes and their possible involvement in neurodegenerative processes.
Original language | English (US) |
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Pages (from-to) | 1114-1123 |
Number of pages | 10 |
Journal | Journal of Cerebral Blood Flow and Metabolism |
Volume | 22 |
Issue number | 9 |
DOIs | |
State | Published - Sep 2002 |
Externally published | Yes |
Keywords
- cDNA macroarray
- Gene expression
- RT-PCR
- TGF-β1 transgenic mice