Transient expression of the chloramphenicol acetyltransferase gene in cultured mosquito cells

Joan E. Durbin, Ann Marie Fallon

Research output: Contribution to journalArticlepeer-review

34 Scopus citations

Abstract

A recombinant plasmid in which the bacterial chloramphenicol acetyltransferase (CAT) gene is under the control of the Drosophila heat-shock protein (hsp) 70 promoter has been introduced into cultured mosquito (Aedes albopictus) cells using 1,5-dimethyl-1,5-diazaundecamethylene polymethobromide (polybrene) and dimethylsulfoxide (DMSO). CAT activity was induced by incubating transfected cells at 37°C, and high levels of enzyme activity were maintained for more than 24 h after the temperature shock. Transfected DNA was maintained in the cells for at least 4 days. These experiments document an effective method for introducing purified DNA into cultured mosquito cells.

Original languageEnglish (US)
Pages (from-to)173-178
Number of pages6
JournalGene
Volume36
Issue number1-2
DOIs
StatePublished - 1985

Bibliographical note

Funding Information:
This work was supportedb y a Basil O’Connor StarterR esearchG rant (No. S-415) fromth eM arch of Dimes Birth DefectsF oundationa nd by a grant from the NIH (A120385)W. e thank Eleanor Kells for typing the manuscript.P lasmid hsp-cat1 was generouslyp rovidedb y Dr. I.B. Dawid.

Keywords

  • Aedes
  • Drosophila
  • Recombinant DNA
  • heat-shock protein promoter
  • polybrene
  • transfection

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