A recombinant plasmid in which the bacterial chloramphenicol acetyltransferase (CAT) gene is under the control of the Drosophila heat-shock protein (hsp) 70 promoter has been introduced into cultured mosquito (Aedes albopictus) cells using 1,5-dimethyl-1,5-diazaundecamethylene polymethobromide (polybrene) and dimethylsulfoxide (DMSO). CAT activity was induced by incubating transfected cells at 37°C, and high levels of enzyme activity were maintained for more than 24 h after the temperature shock. Transfected DNA was maintained in the cells for at least 4 days. These experiments document an effective method for introducing purified DNA into cultured mosquito cells.
Bibliographical noteFunding Information:
This work was supportedb y a Basil O’Connor StarterR esearchG rant (No. S-415) fromth eM arch of Dimes Birth DefectsF oundationa nd by a grant from the NIH (A120385)W. e thank Eleanor Kells for typing the manuscript.P lasmid hsp-cat1 was generouslyp rovidedb y Dr. I.B. Dawid.
- Recombinant DNA
- heat-shock protein promoter