Transplantation of human fetal striatum into a rodent model of Huntington's disease ameliorates locomotor deficits

Lisa L. Pundt; Takeshi Kondoh; John A. Conrad; Walter C Low

doi:10.1016/0168-0102(95)01009-2

Transplantation of human fetal striatum into a rodent model of Huntington's disease ameliorates locomotor deficits

Lisa L. Pundt, Takeshi Kondoh, John A. Conrad, Walter C Low

Neurosurgery

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23 Scopus citations

Abstract

Previous studies have demonstrated that syngeneic transplants of striatal tissue can ameliorate locomotor deficits in rodent models of Huntington's disease (HD). In the present study, we have examined whether human to rat xenografts of fetal striatal tissue can exert a similar recovery of function. Rodents with unilateral striatal lesions were transplanted with human striatal cells from a donor 14 weeks post-conception, and subsequently displayed a progressive decrease in rotational asymmetry in comparison to sham (saline) transplanted animals. Histological analysis revealed acetylcholinesterase (AChE)-positive fibers and NADPH-diaphorase (NADPH-d)-positive neurons within transplanted tissue. These results suggest that human fetal striatum at a gestational age of 14 weeks may potentially be useful as a source of donor tissue for transplantation in the treatment of HD.

Original language	English (US)
Pages (from-to)	415-420
Number of pages	6
Journal	Neuroscience Research
Volume	24
Issue number	4
DOIs	https://doi.org/10.1016/0168-0102(95)01009-2
State	Published - Mar 1996

Bibliographical note

Funding Information:
The authorsw ould like to thank Joan Bailey and Linda King for clericala nd administrativaes sistance, and Mike McPheef or expertg raphicsa ssistanceT.h is work was supportedin part by NIH grantsR 24-HD-30511 and RO1-NS-24464a, nd from Hal Seth, the SUPER Fund, and Ben and Beryl Miller.

Keywords

AChE
Huntington's disease
NADPH-diaphorase
Transplantation

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title = "Transplantation of human fetal striatum into a rodent model of Huntington's disease ameliorates locomotor deficits",

abstract = "Previous studies have demonstrated that syngeneic transplants of striatal tissue can ameliorate locomotor deficits in rodent models of Huntington's disease (HD). In the present study, we have examined whether human to rat xenografts of fetal striatal tissue can exert a similar recovery of function. Rodents with unilateral striatal lesions were transplanted with human striatal cells from a donor 14 weeks post-conception, and subsequently displayed a progressive decrease in rotational asymmetry in comparison to sham (saline) transplanted animals. Histological analysis revealed acetylcholinesterase (AChE)-positive fibers and NADPH-diaphorase (NADPH-d)-positive neurons within transplanted tissue. These results suggest that human fetal striatum at a gestational age of 14 weeks may potentially be useful as a source of donor tissue for transplantation in the treatment of HD.",

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author = "Pundt, {Lisa L.} and Takeshi Kondoh and Conrad, {John A.} and Low, {Walter C}",

note = "Funding Information: The authorsw ould like to thank Joan Bailey and Linda King for clericala nd administrativaes sistance, and Mike McPheef or expertg raphicsa ssistanceT.h is work was supportedin part by NIH grantsR 24-HD-30511 and RO1-NS-24464a, nd from Hal Seth, the SUPER Fund, and Ben and Beryl Miller.",

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AU - Kondoh, Takeshi

AU - Conrad, John A.

AU - Low, Walter C

N1 - Funding Information: The authorsw ould like to thank Joan Bailey and Linda King for clericala nd administrativaes sistance, and Mike McPheef or expertg raphicsa ssistanceT.h is work was supportedin part by NIH grantsR 24-HD-30511 and RO1-NS-24464a, nd from Hal Seth, the SUPER Fund, and Ben and Beryl Miller.

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N2 - Previous studies have demonstrated that syngeneic transplants of striatal tissue can ameliorate locomotor deficits in rodent models of Huntington's disease (HD). In the present study, we have examined whether human to rat xenografts of fetal striatal tissue can exert a similar recovery of function. Rodents with unilateral striatal lesions were transplanted with human striatal cells from a donor 14 weeks post-conception, and subsequently displayed a progressive decrease in rotational asymmetry in comparison to sham (saline) transplanted animals. Histological analysis revealed acetylcholinesterase (AChE)-positive fibers and NADPH-diaphorase (NADPH-d)-positive neurons within transplanted tissue. These results suggest that human fetal striatum at a gestational age of 14 weeks may potentially be useful as a source of donor tissue for transplantation in the treatment of HD.

AB - Previous studies have demonstrated that syngeneic transplants of striatal tissue can ameliorate locomotor deficits in rodent models of Huntington's disease (HD). In the present study, we have examined whether human to rat xenografts of fetal striatal tissue can exert a similar recovery of function. Rodents with unilateral striatal lesions were transplanted with human striatal cells from a donor 14 weeks post-conception, and subsequently displayed a progressive decrease in rotational asymmetry in comparison to sham (saline) transplanted animals. Histological analysis revealed acetylcholinesterase (AChE)-positive fibers and NADPH-diaphorase (NADPH-d)-positive neurons within transplanted tissue. These results suggest that human fetal striatum at a gestational age of 14 weeks may potentially be useful as a source of donor tissue for transplantation in the treatment of HD.

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KW - Huntington's disease

KW - NADPH-diaphorase

KW - Transplantation

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Transplantation of human fetal striatum into a rodent model of Huntington's disease ameliorates locomotor deficits

Abstract

Bibliographical note

Keywords

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