TY - JOUR
T1 - Transplanted Senescent Cells Induce an Osteoarthritis-Like Condition in Mice
AU - Xu, Ming
AU - Bradley, Elizabeth W.
AU - Weivoda, Megan M.
AU - Hwang, Soyun M.
AU - Pirtskhalava, Tamar
AU - Decklever, Teresa
AU - Curran, Geoffry L.
AU - Ogrodnik, Mikolaj
AU - Jurk, Diana
AU - Johnson, Kurt O.
AU - Lowe, Val
AU - Tchkonia, Tamar
AU - Westendorf, Jennifer J.
AU - Kirkland, James L.
N1 - Publisher Copyright:
© The Author 2016. Published by Oxford University Press on behalf of The Gerontological Society of America.
PY - 2017/6/1
Y1 - 2017/6/1
N2 - Osteoarthritis (OA) is the leading form of arthritis in the elderly, causing pain, disability, and immobility. OA has been associated with accumulation of senescent cells in or near joints. However, evidence for a causal link between OA and cellular senescence is lacking. Here, we present a novel senescent cell transplantation model involving injection of small numbers of senescent or nonsenescent cells from the ear cartilage of luciferase-expressing mice into the knee joint area of wild-type mice. By using bioluminescence and 18FDG PET imaging, we could track the injected cells in vivo for more than 10 days. Transplanting senescent cells into the knee region caused leg pain, impaired mobility, and radiographic and histological changes suggestive of OA. Transplanting nonsenescent cells had less of these effects. Thus, senescent cells can induce an OA-like state and targeting senescent cells could be a promising strategy for treating OA.
AB - Osteoarthritis (OA) is the leading form of arthritis in the elderly, causing pain, disability, and immobility. OA has been associated with accumulation of senescent cells in or near joints. However, evidence for a causal link between OA and cellular senescence is lacking. Here, we present a novel senescent cell transplantation model involving injection of small numbers of senescent or nonsenescent cells from the ear cartilage of luciferase-expressing mice into the knee joint area of wild-type mice. By using bioluminescence and 18FDG PET imaging, we could track the injected cells in vivo for more than 10 days. Transplanting senescent cells into the knee region caused leg pain, impaired mobility, and radiographic and histological changes suggestive of OA. Transplanting nonsenescent cells had less of these effects. Thus, senescent cells can induce an OA-like state and targeting senescent cells could be a promising strategy for treating OA.
KW - Bioluminescence imaging
KW - Cell transplantation
KW - Fluorodeoxyglucose
KW - Mechanical allodynia
KW - Senolytics
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U2 - 10.1093/gerona/glw154
DO - 10.1093/gerona/glw154
M3 - Article
C2 - 27516624
AN - SCOPUS:85027520811
SN - 1079-5006
VL - 72
SP - 780
EP - 785
JO - The journals of gerontology. Series A, Biological sciences and medical sciences
JF - The journals of gerontology. Series A, Biological sciences and medical sciences
IS - 6
ER -