Sequencing batch reactors (SBRs) were used to degrade phenol compounds efficiently; however, most investigations on such reactors have focused on fresh rather than saline wastewaters, even though saline effluents containing phenolic compounds are generated by many industries. This study assessed the performance of the aerobic SBR process in the removal of phenol as the sole substrate under conditions of high salinity. A flexible concentration of phenol could be completely degraded in SBR process, varied from 400mgl-1 to 1200mgl-1 at the existing of 80gl-1 NaCl. The value of specific oxygen uptake rate (SOUR) suggested that the exiting bacteria could adapt to the phenol and salt conditions well at each stage. Cloning and sequencing of the 16S rRNA showed that the acclimated active sludge included two dominant genera: Pseudomonas and Alcaligenes. PCR detection of the functional genes suggested that phenol hydroxylase (Lph), catechol 1,2-dioxygenase (C12O), and catechol 2,3-dioxygenase (C23O) were active in the phenol-degradation process. Real-time PCR showed that the phenol-degrading bacteria comprised 63.3% of the total bacterial community.
Bibliographical noteFunding Information:
Supported jointly by National Natural Science Foundation of China ( 51378208 , 41003031 , 41273109 ), Specialized Research Fund for the Doctoral Program of Higher Education ( 20110074130002 ), Program for New Century Excellent Talents in University ( NCET-13-0797 ), Fok Ying Tung Education Foundation (141077), Shanghai Rising-Star Program ( 12QA1400800 ), Innovation Program of Shanghai Municipal Education Commission ( 14ZZ059 ), Fundamental Research Funds for the Central Universities ( 222201313008 ). We also would like to thank the anonymous referees for their helpful comments on this paper.
- Moderate halophiles
- Phenol-laden wastewater
- Sequencing batch reactor