TY - JOUR
T1 - TRH-like antidepressant peptide, pyroglutamyltyroslyprolineamide, occurs in rat brain
AU - Pekary, Eugene A.
AU - Faull, Kym F.
AU - Paulson, Mark
AU - Lloyd, Robert L
AU - Sattin, Albert
N1 - Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2005/9
Y1 - 2005/9
N2 - We have previously reported the occurrence of pGlu-Glu-Pro-NH2 (Glu-TRH, EEP), Val-TRH, Tyr-TRH, Leu-TRH, Phe-TRH, and Trp-TRH in rat brain using a combination of HPLC and radioimmunoassays with antibodies that cross-react with the general structure pGlu-X-Pro-NH2 where 'X' maybe any amino acid residue (Peptides 2004; 25:647). This new family of TRH-like peptides, along with TRH (pGlu-His-PrO-NH2), has neuroprotective, anticonvulsant, antidepressant, euphoric, anti-amnesic, and analeptic effects. We now report that a combination of affinity chromatography using a rabbit antibody specific for Tyr-TRH and Phe-TRH, along with HPLC and tandem mass spectrometry operating in the multiple reaction monitoring (MRM) mode, provide conclusive evidence for the presence of Tyr-TRH in rat brain. Furthermore, synthetic Tyr-TRH is active in the Porsolt Swim Test suggesting that it is a fourth member of this family of in vivo neuroregulatory agents that have psychopharmacotherapeutic properties.
AB - We have previously reported the occurrence of pGlu-Glu-Pro-NH2 (Glu-TRH, EEP), Val-TRH, Tyr-TRH, Leu-TRH, Phe-TRH, and Trp-TRH in rat brain using a combination of HPLC and radioimmunoassays with antibodies that cross-react with the general structure pGlu-X-Pro-NH2 where 'X' maybe any amino acid residue (Peptides 2004; 25:647). This new family of TRH-like peptides, along with TRH (pGlu-His-PrO-NH2), has neuroprotective, anticonvulsant, antidepressant, euphoric, anti-amnesic, and analeptic effects. We now report that a combination of affinity chromatography using a rabbit antibody specific for Tyr-TRH and Phe-TRH, along with HPLC and tandem mass spectrometry operating in the multiple reaction monitoring (MRM) mode, provide conclusive evidence for the presence of Tyr-TRH in rat brain. Furthermore, synthetic Tyr-TRH is active in the Porsolt Swim Test suggesting that it is a fourth member of this family of in vivo neuroregulatory agents that have psychopharmacotherapeutic properties.
KW - Affinity chromatography
KW - HPLC
KW - Mass spectrometry
KW - TRH-like peptides
UR - http://www.scopus.com/inward/record.url?scp=25144437513&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=25144437513&partnerID=8YFLogxK
U2 - 10.1002/jms.904
DO - 10.1002/jms.904
M3 - Article
C2 - 16124040
AN - SCOPUS:25144437513
SN - 1076-5174
VL - 40
SP - 1232
EP - 1236
JO - Journal of Mass Spectrometry
JF - Journal of Mass Spectrometry
IS - 9
ER -