Three new aminolipopeptides, designated trichoderins A (1), A1 (2), and B (3), were isolated from a culture of marine sponge-derived fungus of Trichoderma sp. as anti-mycobacterial substances with activity against active and dormant bacilli. The chemical structures of trichoderins were determined on the basis of spectroscopic study. Trichoderins showed potent anti-mycobacterial activity against Mycobacterium smegmatis, Mycobacterium bovis BCG, and Mycobacterium tuberculosis H37Rv under standard aerobic growth conditions as well as dormancy-inducing hypoxic conditions, with MIC values in the range of 0.02-2.0 μg/mL.
Bibliographical noteFunding Information:
The authors thank Ms. Salona Moodley, Mr. Yushir R. Maharaj, Ms. Karnishree Govender (University of KwaZulu Natal) for excellent technical assistance. A.D.B. was supported by a Grant from the Helen Hay Whitney Foundation . This study was financially supported by the Hoh-ansha Foundation , the Uehara Memorial Foundation , JSPS-South Africa (NRF) Joint Research Program , and Grant-in-Aid for scientific research from the Ministry of Education, Culture, Sports, Science and Technology of Japan .
- Anti-mycobacterial activity
- Trichoderma sp.