Two linear plasmids in mitochondria of Fusarium solani f. sp. cucurbitae

Deborah A. Samac, Sally A. Leong

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

Two linear plasmid-like DNAs designated pFSC1 (9.2 kbp) and pFSC2 (8.3 kbp) were found in an isolate of the plant pathogenic fungus Fusarium solani f. sp. cucurbitae race 1. The plasmids were maternally inherited and copurified with mitochondrial DNA obtained from a mitochondria-enriched cell fraction suggesting that they are located in mitochondria. The plasmids did not share extensive sequence similarity. No homology was detected between either plasmid and the nuclear or mitochondrial genome when cloned plasmids were used as probes in Southern hybridization analyses. The fungus was cured of plasmids by ethidium bromide treatment. Compared to the plasmid-containing isolate, plasmid-cured derivatives had reduced pathogenicity on a susceptible plant host, Cucurbita maxima "Pink Banana.".

Original languageEnglish (US)
Pages (from-to)57-67
Number of pages11
JournalPlasmid
Volume19
Issue number1
DOIs
StatePublished - Jan 1988

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