Type of Antibody Elevated by 5-Fluoro-2-deoxyuridine and Endotoxin During the Primary Response of the Mouse

Mice stimulated with human gamma globulin formed antibody which was mercaptoethanol sensitive and assumed to be of the 19S variety. Incorporation of bacterial endotoxin with the human gamma globulin stimulated antibody to appear earlier and to higher levels. This increased antibody response was due both to production of mercaptoethanol sensitive antibody and mercaptoethanol insensitive antibody. Injection of 5-fluoro-2-deoxyuridine one hour before the human gamma globulin also resulted in early and enhanced levels of mercaptoethanol sensitive antibody, with an increase in the level of low molecular weight antibody appearing later. Similarly, C58 mice receiving endo-toxin and BGG formed antibody earlier and to greater levels than did those receiving BGG alone. The increased antibody production in this strain also was due to both mercaptoethanol sensitive and insensitive antibody.