Ultrastable combined atomic force and total internal fluorescence microscope

H. Gumpp, S. W. Stahl, M. Strackharn, E. M. Puchner, H. E. Gaub

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49 Scopus citations

Abstract

Combining atomic force microscope (AFM) with other microscopy techniques has expanded the range of potential applications for single molecule investigations dramatically. Particularly hybrid instruments with total internal reflection fluorescence (TIRF) excitation have opened new routes in life sciences. Here we present a novel design for such a hybrid microscope, which overcomes the limitations of conventional combinations caused by their limited mechanical stability. A thorough analysis of the noise spectra and a comparison of the different designs and the different operation modes are given. With this instrument we demonstrate single molecule manipulation by AFM and simultaneous TIRF imaging.

Original languageEnglish (US)
Article number063704
JournalReview of Scientific Instruments
Volume80
Issue number6
DOIs
StatePublished - 2009

Bibliographical note

Funding Information:
We thank Ferdinand Kühner, Matthias Erdmann, and Jan Opfer for helpful discussions. This work was supported by the Nanosystems Initiative Munich (NIM) and the Stiftung Volkswagenwerk.

Copyright:
Copyright 2012 Elsevier B.V., All rights reserved.

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