Abstract
Altered expression of long noncoding RNAs (lncRNAs) associated with human carcinogenesis. We performed a cDNA microarray analysis of lncRNA expression in 12 cases of nasopharyngeal carcinoma (NPC) and 4 non-tumor nasopharyngeal epitheliums. One lncRNA, actin filament associated protein 1 antisense RNA1 (AFAP1- AS1), was identified and selected for further study. AFAP1-AS1 expression was upregulated in NPC and associated with NPC metastasis and poor prognosis. In vitro experiments demonstrated that AFAP1-AS1 knockdown significantly inhibited the NPC cell migration and invasive capability. AFAP1-AS1 knockdown also increased AFAP1 protein expression. Proteomic and bioinformatics analyses suggested that AFAP1-AS1 affected the expression of several small GTPase family members and molecules in the actin cytokeratin signaling pathway. AFAP1-AS1 promoted cancer cell metastasis via regulation of actin filament integrity. AFAP1-AS1 might be a potential novel marker that can predict cancer patient prognosis and as a potential therapeutic target for NPC.
Original language | English (US) |
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Pages (from-to) | 20404-20418 |
Number of pages | 15 |
Journal | Oncotarget |
Volume | 6 |
Issue number | 24 |
DOIs | |
State | Published - 2015 |
Keywords
- AFAP1 antisense RNA1 (AFAP1-AS1)
- Long non-coding RNA (LncRNA)
- Metastasis
- Nasopharyngeal carcinoma (NPC)
- Prognosis