Upregulation of jasmonate biosynthesis and jasmonate-responsive genes in rice leaves in response to a bacterial pathogen mimic

Ashish Ranjan, Jyothilakshmi Vadassery, Hitendra Kumar Patel, Alok Pandey, Ramesh Palaparthi, Axel Mithöfer, Ramesh V. Sonti

Research output: Contribution to journalArticlepeer-review

Abstract

Xanthomonas oryzae pv. oryzae, the causal agent of bacterial blight of rice, secretes several cell wall degrading enzymes including cellulase (ClsA) and lipase/esterase (LipA). Prior treatment of rice leaves with purified cell wall degrading enzymes such as LipA can confer enhanced resistance against subsequent X. oryzae pv. oryzae infection. To understand LipA-induced rice defense responses, microarray analysis was performed 12 h after enzyme treatment of rice leaves. This reveals that 867 (720 upregulated and 147 downregulated) genes are differentially regulated (≥2-fold). A number of genes involved in defense, stress, signal transduction, and catabolic processes were upregulated while a number of genes involved in photosynthesis and anabolic processes were downregulated. The microarray data also suggested upregulation of jasmonic acid (JA) biosynthetic and JA-responsive genes. Estimation of various phytohormones in LipA-treated rice leaves demonstrated a significant increase in the level of JA-Ile (a known active form of JA) while the levels of other phytohormones were not changed significantly with respect to buffer-treated control. This suggests a role for JA-Ile in cell wall damage induced innate immunity. Furthermore, a comparative analysis of ClsA- and LipA-induced rice genes has identified key rice functions that might be involved in elaboration of damage-associated molecular pattern (DAMP)-induced innate immunity.

Original languageEnglish (US)
Pages (from-to)363-373
Number of pages11
JournalFunctional and Integrative Genomics
Volume15
Issue number3
DOIs
StatePublished - May 1 2015
Externally publishedYes

Keywords

  • Cell wall degrading enzyme
  • Damage-associated molecular patterns (DAMPs)
  • Jasmonic acid
  • Plant defense responses
  • Rice
  • Xanthomonas oryzae

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