TY - JOUR
T1 - Use of microarray analysis to dissect the plant defense response.
AU - Glazebrook, Jane
PY - 2007
Y1 - 2007
N2 - Microarray analysis is a technology that allows simultaneous measurement of the messenger RNA levels of thousands of genes. There are several different technology platforms in use, including oligo arrays synthesized directly on the underlying substrate, and spotted arrays produced by applying oligonucleotides or other nucleic acids to glass slides. The advantages of various platforms are discussed. Analysis of the large data sets produced from microarray experiments requires the application of statistical methods to define significant differences in gene expression, and computerized algorithms for pattern recognition. Early applications of microarray analysis to studies of disease resistance have led to recognition of the large numbers of genes that respond to infection, insights into the nature of gene-for-gene resistance, efforts to model the topology of the signaling network controlling inducible defense responses, and identification of promoter elements associated with particular expression patterns.
AB - Microarray analysis is a technology that allows simultaneous measurement of the messenger RNA levels of thousands of genes. There are several different technology platforms in use, including oligo arrays synthesized directly on the underlying substrate, and spotted arrays produced by applying oligonucleotides or other nucleic acids to glass slides. The advantages of various platforms are discussed. Analysis of the large data sets produced from microarray experiments requires the application of statistical methods to define significant differences in gene expression, and computerized algorithms for pattern recognition. Early applications of microarray analysis to studies of disease resistance have led to recognition of the large numbers of genes that respond to infection, insights into the nature of gene-for-gene resistance, efforts to model the topology of the signaling network controlling inducible defense responses, and identification of promoter elements associated with particular expression patterns.
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M3 - Article
C2 - 17172750
AN - SCOPUS:39049177125
SN - 1064-3745
VL - 354
SP - 121
EP - 130
JO - Methods in molecular biology (Clifton, N.J.)
JF - Methods in molecular biology (Clifton, N.J.)
ER -