Use of Petunia inflata as a model for the study of solanaceous type self-incompatibility

Peter E. Dowd, Andrew G. McCubbin, Xi Wang, Joseph A. Verica, Tatsuya Tsukamoto, Toshio Ando, Teh Hui Kao

Research output: Contribution to journalArticlepeer-review

29 Scopus citations

Abstract

Petunia inflata has been used as a model to study solanaceous type self-incompatibility (SI), an RNase-mediated self/non-self recognition mechanism. Pistil proteins that co-segregate with S-alleles (termed S-proteins or S-RNases) were identified, and their role in SI was demonstrated by gain-of-function and loss-of-function experiments. Several lines of evidence strongly suggest that the S-locus contains another gene, termed the pollen S-gene, whose allelic products presumably interact with S-RNases to trigger the SI response. Thus, ultimate understanding of the molecular/biochemical basis of SI requires the identification of the pollen S-gene. A number of approaches that have been used to accomplish this objective are discussed. Differential display and subtractive hybridization have yielded the most promising results: cDNAs corresponding to 13 pollen-expressed genes that lie within 1 cM of the S-RNase gene have been identified. They will be used as markers to identify large S-linked genomic fragments which will then be examined for the presence of the pollen S-gene by plant transformation. (C) 2000 Annals of Botany Company.

Original languageEnglish (US)
Pages (from-to)87-93
Number of pages7
JournalAnnals of Botany
Volume85
Issue numberSUPPL. A
DOIs
StatePublished - 2000

Bibliographical note

Funding Information:
This work was supported by a grant to T.-h.K. from the US National Science Foundation. P.E.D. was supported by a pre-doctoral fellowship award from the US National Institutes of Health.

Keywords

  • Petunia inflata
  • Pollen-pistil interactions
  • S-R Nases
  • S-linked cDNA markers
  • Self-incompatibility

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