The tomato PROCERA gene encodes a DELLA protein, and loss-of-function mutations derepress growth. We used CRISPR/Cas9 and a single guide RNAs (sgRNA) to target mutations to the PROCERA DELLA domain, and recovered several loss-of-function mutations and a dominant dwarf mutation that carries a deletion of one amino acid in the DELLA domain. This is the first report of a dominant dwarf PROCERA allele. This allele retains partial responsiveness to exogenously applied gibberellin. Heterozygotes show an intermediate phenotype at the seedling stage, but adult heterozygotes are as dwarfed as homozygotes.
Bibliographical noteFunding Information:
This work was supported by grants from Bill and Melinda Gates Foundation (Grand Challenges Exploration, grant number OPP1060026), Biotechnology and Biological Sciences Research Council (BBSRC) BB/L009293/1), US Israel Binational Agriculture Research and Development Fund to N.O. (grant no. US-4813-15C) and Gatsby Charitable Foundation. We are also grateful to Liz Hughes and Neil Stammars for their technical help.
© 2018 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd.
- gibberellic acid