TY - JOUR
T1 - Using crude whole-genome assemblies of Neisseria gonorrhoeae as a platform for strain analysis
T2 - Clonal spread of gonorrhea infection in saskatchewan, Canada
AU - Vidovic, Sinisa
AU - Caron, Carolyn
AU - Taheri, Ali
AU - Thakur, Sidharath D.
AU - Read, Timothy D.
AU - Kusalik, Anthony
AU - Dillon, Jo Anne R
N1 - Publisher Copyright:
© 2014, American Society for Microbiology. All Rights Reserved.
PY - 2014/10/1
Y1 - 2014/10/1
N2 - Using crude whole-genome assemblies, we analyzed 25 isolates of Neisseria gonorrhoeae by using a high-resolution single nucleotide polymorphism (SNP) approach for nine housekeeping genes, characterizing penA alleles, and antimicrobial susceptibility phenotypes coupled with population structure analysis. Two clonal complexes, characterized by their spatial and geographical persistence, were identified. In addition, the clonal spread of penicillin-resistant/intermediate phenotypes and a novel introduction of the azithromycin resistance phenotype in Saskatchewan, Canada, were ascertained using this method.
AB - Using crude whole-genome assemblies, we analyzed 25 isolates of Neisseria gonorrhoeae by using a high-resolution single nucleotide polymorphism (SNP) approach for nine housekeeping genes, characterizing penA alleles, and antimicrobial susceptibility phenotypes coupled with population structure analysis. Two clonal complexes, characterized by their spatial and geographical persistence, were identified. In addition, the clonal spread of penicillin-resistant/intermediate phenotypes and a novel introduction of the azithromycin resistance phenotype in Saskatchewan, Canada, were ascertained using this method.
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U2 - 10.1128/JCM.01502-14
DO - 10.1128/JCM.01502-14
M3 - Article
C2 - 25056324
AN - SCOPUS:84907168910
SN - 0095-1137
VL - 52
SP - 3772
EP - 3776
JO - Journal of clinical microbiology
JF - Journal of clinical microbiology
IS - 10
ER -