TY - JOUR
T1 - Using dominant eigenvalue analysis to predict formation of alternans in the heart
AU - Kakade, Virendra
AU - Zhao, Xiaopeng
AU - Tolkacheva, Elena G.
PY - 2013/11/22
Y1 - 2013/11/22
N2 - Ventricular fibrillation at the whole heart level is often preceded by the alternation of action potential duration (APD), i.e., alternans, at the cellular level. As proven in many experiments, traditional approaches based on the slope of the restitution curve have not been successful in predicting alternans formation. Recently, a technique has been theoretically developed based on dominant eigenvalue analysis to predict alternans formation in isolated cardiac myocytes. Here, we aimed to demonstrate that this technique can be applied to predict alternans formation at the whole heart level. Optical mapping was performed in Langendorff-perfused hearts from New Zealand white rabbits (n = 4), which were paced at decreasing basic cycle lengths to introduce APD alternans. In each heart, the basic cycle length corresponding to the local onset of alternans, Bonset, was determined and two regions of the heart were identified at Bonset: one region which exhibited alternans (1:1alt) and one which did not (1:1). Corresponding two-dimensional eigenvalue (λ) maps were generated using principal component analysis by analyzing action potentials after short perturbations from the steady state, and mean eigenvalues (λ̄) were calculated separately for the 1:1 and 1:1alt regions. We demonstrated that λ̄ calculated at Bonset was significantly different (p<0.05) between the two regions. Our results suggest that this dominant eigenvalue technique can be used to successfully predict the local alternans formation in the heart.
AB - Ventricular fibrillation at the whole heart level is often preceded by the alternation of action potential duration (APD), i.e., alternans, at the cellular level. As proven in many experiments, traditional approaches based on the slope of the restitution curve have not been successful in predicting alternans formation. Recently, a technique has been theoretically developed based on dominant eigenvalue analysis to predict alternans formation in isolated cardiac myocytes. Here, we aimed to demonstrate that this technique can be applied to predict alternans formation at the whole heart level. Optical mapping was performed in Langendorff-perfused hearts from New Zealand white rabbits (n = 4), which were paced at decreasing basic cycle lengths to introduce APD alternans. In each heart, the basic cycle length corresponding to the local onset of alternans, Bonset, was determined and two regions of the heart were identified at Bonset: one region which exhibited alternans (1:1alt) and one which did not (1:1). Corresponding two-dimensional eigenvalue (λ) maps were generated using principal component analysis by analyzing action potentials after short perturbations from the steady state, and mean eigenvalues (λ̄) were calculated separately for the 1:1 and 1:1alt regions. We demonstrated that λ̄ calculated at Bonset was significantly different (p<0.05) between the two regions. Our results suggest that this dominant eigenvalue technique can be used to successfully predict the local alternans formation in the heart.
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U2 - 10.1103/PhysRevE.88.052716
DO - 10.1103/PhysRevE.88.052716
M3 - Article
C2 - 24329305
AN - SCOPUS:84889640437
SN - 1539-3755
VL - 88
JO - Physical Review E - Statistical, Nonlinear, and Soft Matter Physics
JF - Physical Review E - Statistical, Nonlinear, and Soft Matter Physics
IS - 5
M1 - 052716
ER -