Application de la microfluidique de gouttes à la conception d'un appareil de PCR en flux continu

Translated title of the contribution: Using droplet microfluidics to design a continuous flow PCR system

Max Chabert, Kevin D. Dorfman, Jean Louis Viovy

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

The design of a two-phase-flow (water in oil) microfluidic PCR svstem led us to solve different problems in order to achieve DNA amplification: droplet train instability, cross contamination between droplets, being able to coalesce two droplets without direct contact with them. After a short explanation of the PCR principle, the experimental conditions used to achieve droplets train stability and avoid contamination between droplets will be presented, as well as the amplification results obtained. We will then present a system based on the electrocoalescence principle which allows to coalesce without direct contact two water droplets inside the capillary.

Translated title of the contributionUsing droplet microfluidics to design a continuous flow PCR system
Original languageFrench
Pages (from-to)51-56
Number of pages6
JournalHouille Blanche
Issue number5
DOIs
StatePublished - Nov 21 2006

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