Abstract
Embryonic stem cells, which can differentiate into insulin-producing cells and express pancreas specific markers during development in vitro, provide a model for studying pancreatic development. Current models of pancreatic development are based on animal models, including zebrafish, chicken and mouse. Genetic manipulations in the mouse have been a major source of information to date.More recently, in vitro differentiation of mouse embryonic stem cells has been used to allow both environmental and genetic manipulation during differentiation.While gene expression during in vitro differentiation appears to reproduce patterns found in vivo, comparison of mouse and human embryonic stem cell differentiation has demonstrated differences between regulation of mouse and human development. In this chapter, we review several studies on ES cell differentiation into insulinproducing cells in vitro and analysis of pancreatic-specific gene expression during differentiation, and discuss the potential of using human ES cells as a model of human pancreatic development.
| Original language | English (US) |
|---|---|
| Title of host publication | Stem Cells and Regenerative Medicine |
| Publisher | World Scientific Publishing Co. |
| Pages | 27-44 |
| Number of pages | 18 |
| ISBN (Electronic) | 9789812775771 |
| ISBN (Print) | 9812775765, 9789812775764 |
| DOIs | |
| State | Published - Jan 1 2008 |