TY - JOUR
T1 - Using knockout and transgenic mice to study neurophysiology and behavior
AU - Picciotto, Marina R.
AU - Wickman, Kevin
PY - 1998/10
Y1 - 1998/10
N2 - Reverse genetics, in which detailed knowledge of a gene of interest permits in vivo modification of its expression or function, provides a powerful method for examining the physiological relevance of any protein. Transgenic and knockout mouse models are particularly useful for studies of complex neurobiological problems. The primary aims of this review are to familiarize the nonspecialist with the techniques and limitations of mouse mutagenesis, to describe new technologies that may overcome these limitations, and to illustrate, using representative examples from the literature, some of the ways in which genetically altered mice have been used to analyze central nervous system function. The goal is to provide the information necessary to evaluate critically studies in which mutant mice have been used to study neurobiological problems.
AB - Reverse genetics, in which detailed knowledge of a gene of interest permits in vivo modification of its expression or function, provides a powerful method for examining the physiological relevance of any protein. Transgenic and knockout mouse models are particularly useful for studies of complex neurobiological problems. The primary aims of this review are to familiarize the nonspecialist with the techniques and limitations of mouse mutagenesis, to describe new technologies that may overcome these limitations, and to illustrate, using representative examples from the literature, some of the ways in which genetically altered mice have been used to analyze central nervous system function. The goal is to provide the information necessary to evaluate critically studies in which mutant mice have been used to study neurobiological problems.
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U2 - 10.1152/physrev.1998.78.4.1131
DO - 10.1152/physrev.1998.78.4.1131
M3 - Review article
C2 - 9790572
AN - SCOPUS:0031722020
SN - 0031-9333
VL - 78
SP - 1131
EP - 1163
JO - Physiological Reviews
JF - Physiological Reviews
IS - 4
ER -