Using Oct4:MerCreMer lineage tracing to monitor endogenous Oct4 expression during the reprogramming of fibroblasts into induced pluripotent stem cells (iPSCs)

Lucas V. Greder, Jason Post, James R. Dutton

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Scopus citations

Abstract

The reprogramming of somatic cells into induced pluripotent stem cells (iPSCs) using a combination of defined transcription factors has become one of the most widely used techniques in stem cell biology. A critical, early event in iPSC reprogramming is the induction of the endogenous transcription factor network that maintains pluripotency in iPSCs. Here we describe using a transgenic, conditional Oct4-Cre construct to investigate the spatial and temporal induction of endogenous Oct4 expression during the reprogramming of mouse fibroblasts into iPS cells.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages97-110
Number of pages14
DOIs
StatePublished - Jan 1 2016

Publication series

NameMethods in Molecular Biology
Volume1357
ISSN (Print)1064-3745

Keywords

  • IPSC
  • Induced pluripotent stem cell
  • Lineage tracing
  • Oct4
  • Oct4-Cre
  • Reprogramming

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