Abstract
The reprogramming of somatic cells into induced pluripotent stem cells (iPSCs) using a combination of defined transcription factors has become one of the most widely used techniques in stem cell biology. A critical, early event in iPSC reprogramming is the induction of the endogenous transcription factor network that maintains pluripotency in iPSCs. Here we describe using a transgenic, conditional Oct4-Cre construct to investigate the spatial and temporal induction of endogenous Oct4 expression during the reprogramming of mouse fibroblasts into iPS cells.
Original language | English (US) |
---|---|
Title of host publication | Methods in Molecular Biology |
Publisher | Humana Press Inc. |
Pages | 97-110 |
Number of pages | 14 |
DOIs | |
State | Published - Jan 1 2016 |
Publication series
Name | Methods in Molecular Biology |
---|---|
Volume | 1357 |
ISSN (Print) | 1064-3745 |
Bibliographical note
Publisher Copyright:© Springer Science+Business Media New York 2014.
Keywords
- IPSC
- Induced pluripotent stem cell
- Lineage tracing
- Oct4
- Oct4-Cre
- Reprogramming