Utility and evolution of cytochrome b in insects

R. B. Simmons; S. J. Weller

doi:10.1006/mpev.2001.0958

Utility and evolution of cytochrome b in insects

R. B. Simmons, S. J. Weller

Entomology

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126 Scopus citations

Abstract

Cytochrome b (cyt-b) is widely used in molecular phylogenetic studies of vertebrate, but not invertebrate, taxa. To determine whether this situation is an historical accident or reflects the utility of cyt-b, we compared the abilities of cyt-b, COI, and one nuclear ribosomal gene region (D1 of 28S) to recover intergeneric relationships within the tiger moth tribes Ctenuchini and Euchromiini. Additionally, we compared the rate of sequence and amino acid evolution of cyt-b across insects. Cytochrome b had the same level of sequence variation and A/T bias as COI, but was less useful for recovering intergeneric relationships. The total evidence tree casts doubt on the traditional taxonomy of the group. For the class Insecta, we found that functional conservation of amino acids occurs for the same regions as those found in vertebrates with the exception of Mallophaga (lice). Lice have an accelerated rate of nonsynonymous substitutions. Accelerated rate of cyt-b nucleotide and amino acid evolution in Apidae (bees) may be correlated with increased metabolic rates associated with facultative endothermy (= heterothermy).

Original language	English (US)
Pages (from-to)	196-210
Number of pages	15
Journal	Molecular Phylogenetics and Evolution
Volume	20
Issue number	2
DOIs	https://doi.org/10.1006/mpev.2001.0958
State	Published - 2001

Bibliographical note

Funding Information:
We thank the following for their comments: two anonymous reviewers, A. Kessen, A. Prather, S. Lanyon, and R. Zink. S. Farrell aided in laboratory work and data collection. J. Moe assisted with Fig. 1. We thank R. Holzenthal (University of Minnesota, Insect Collection) for providing museum material. Simmons’ fieldwork was funded by a Theodore Roosevelt grant (American Museum of Natural History) and a Dayton-Wilkie grant (Bell Museum of Natural History). Additional funding was provided by NSF Doctoral dissertation improvement grant, DEB-9706192 (R. Simmons) and NSF DEB-9306755 and Grant-in-Aid, University of Minnesota (S. Weller).

Keywords

Cytochrome b
Insecta
Metabolic rate hypothesis

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abstract = "Cytochrome b (cyt-b) is widely used in molecular phylogenetic studies of vertebrate, but not invertebrate, taxa. To determine whether this situation is an historical accident or reflects the utility of cyt-b, we compared the abilities of cyt-b, COI, and one nuclear ribosomal gene region (D1 of 28S) to recover intergeneric relationships within the tiger moth tribes Ctenuchini and Euchromiini. Additionally, we compared the rate of sequence and amino acid evolution of cyt-b across insects. Cytochrome b had the same level of sequence variation and A/T bias as COI, but was less useful for recovering intergeneric relationships. The total evidence tree casts doubt on the traditional taxonomy of the group. For the class Insecta, we found that functional conservation of amino acids occurs for the same regions as those found in vertebrates with the exception of Mallophaga (lice). Lice have an accelerated rate of nonsynonymous substitutions. Accelerated rate of cyt-b nucleotide and amino acid evolution in Apidae (bees) may be correlated with increased metabolic rates associated with facultative endothermy (= heterothermy).",

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Utility and evolution of cytochrome b in insects

Abstract

Bibliographical note

Keywords

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