Vaccination Against Lawsonia intracellularis Decreases Shedding of Salmonella enterica serovar Typhimurium in Co-Infected Pigs and Alters the Gut Microbiome

Fernando L.L. Leite; Randall S. Singer; Tonya Ward; Connie J. Gebhart; Richard E. Isaacson

doi:10.1038/s41598-018-21255-7

Vaccination Against Lawsonia intracellularis Decreases Shedding of Salmonella enterica serovar Typhimurium in Co-Infected Pigs and Alters the Gut Microbiome

Fernando L.L. Leite, Randall S. Singer, Tonya Ward, Connie J. Gebhart, Richard E. Isaacson

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

Salmonella enterica serovar Typhimurium continues to be a major cause of foodborne illness worldwide and pork can serve as a source of infection. Co-infection of S. enterica with Lawsonia intracellularis, a common intestinal pathogen of swine, has been found as risk factor for increased S. enterica shedding. The objective of this study was to investigate if vaccination against L. intracellularis could lead to decreased S. Typhimurium shedding. To test this hypothesis, pigs were challenged with either S. Typhimurium or S. Typhimurium and L. intracellularis, with and without L. intracellularis vaccination (n = 9 per group). A non-challenged group served as a negative control. Vaccination decreased the shedding of S. Typhimurium in co-infected animals by 2.12 log₁₀ organisms per gram of feces at 7 days post infection. Analysis of the microbiome showed that vaccination led to changes in the abundance of Clostridium species, including Clostridium butyricum, in addition to other compositional changes that may explain the protection mediated against S. Typhimurium. These results indicate that vaccination against L. intracellularis in co-infected herds may provide a new tool to increase food safety by helping to prevent S. enterica without the need for antibiotics.

Original language	English (US)
Article number	2857
Journal	Scientific reports
Volume	8
Issue number	1
DOIs	https://doi.org/10.1038/s41598-018-21255-7
State	Published - Dec 1 2018

Bibliographical note

Funding Information:
We would like to thank Trevor Gould from the University of Minnesota Informatics Institute (UMII) for his assistance in generating the OTU table for analysis and Aaron Rhendal for his assistance in statistical analysis of shedding data. For help in collection of samples and technical assistance we wish to thank: Fabian Chamba, Talita Resende, Yonghyan Kim, Luiza Roos, Jorge Garrido, Britta Wass, Kyrstin Danielson, Grace Moores and Jackelyn Goldschmidt. We would like to thank Dana Beckler for preparation of the Lawsonia intracellularis culture and assistance in challenge of animals. We would also like to thank the University of Minnesota Genomics Center for sequencing of samples for microbiome analysis. This work was supported by USDA-AFRI grant 1005423.

Publisher Copyright:
© 2018 The Author(s).

Access

10.1038/s41598-018-21255-7

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5809363

OpenUrl availability

Full text

Cite this

@article{8df33af4ac434e93afa48fb87feb6027,

title = "Vaccination Against Lawsonia intracellularis Decreases Shedding of Salmonella enterica serovar Typhimurium in Co-Infected Pigs and Alters the Gut Microbiome",

abstract = "Salmonella enterica serovar Typhimurium continues to be a major cause of foodborne illness worldwide and pork can serve as a source of infection. Co-infection of S. enterica with Lawsonia intracellularis, a common intestinal pathogen of swine, has been found as risk factor for increased S. enterica shedding. The objective of this study was to investigate if vaccination against L. intracellularis could lead to decreased S. Typhimurium shedding. To test this hypothesis, pigs were challenged with either S. Typhimurium or S. Typhimurium and L. intracellularis, with and without L. intracellularis vaccination (n = 9 per group). A non-challenged group served as a negative control. Vaccination decreased the shedding of S. Typhimurium in co-infected animals by 2.12 log10 organisms per gram of feces at 7 days post infection. Analysis of the microbiome showed that vaccination led to changes in the abundance of Clostridium species, including Clostridium butyricum, in addition to other compositional changes that may explain the protection mediated against S. Typhimurium. These results indicate that vaccination against L. intracellularis in co-infected herds may provide a new tool to increase food safety by helping to prevent S. enterica without the need for antibiotics.",

author = "Leite, {Fernando L.L.} and Singer, {Randall S.} and Tonya Ward and Gebhart, {Connie J.} and Isaacson, {Richard E.}",

note = "Funding Information: We would like to thank Trevor Gould from the University of Minnesota Informatics Institute (UMII) for his assistance in generating the OTU table for analysis and Aaron Rhendal for his assistance in statistical analysis of shedding data. For help in collection of samples and technical assistance we wish to thank: Fabian Chamba, Talita Resende, Yonghyan Kim, Luiza Roos, Jorge Garrido, Britta Wass, Kyrstin Danielson, Grace Moores and Jackelyn Goldschmidt. We would like to thank Dana Beckler for preparation of the Lawsonia intracellularis culture and assistance in challenge of animals. We would also like to thank the University of Minnesota Genomics Center for sequencing of samples for microbiome analysis. This work was supported by USDA-AFRI grant 1005423. Publisher Copyright: {\textcopyright} 2018 The Author(s).",

year = "2018",

month = dec,

day = "1",

doi = "10.1038/s41598-018-21255-7",

language = "English (US)",

volume = "8",

journal = "Scientific reports",

issn = "2045-2322",

publisher = "Nature Publishing Group",

number = "1",

}

TY - JOUR

T1 - Vaccination Against Lawsonia intracellularis Decreases Shedding of Salmonella enterica serovar Typhimurium in Co-Infected Pigs and Alters the Gut Microbiome

AU - Leite, Fernando L.L.

AU - Singer, Randall S.

AU - Ward, Tonya

AU - Gebhart, Connie J.

AU - Isaacson, Richard E.

N1 - Funding Information: We would like to thank Trevor Gould from the University of Minnesota Informatics Institute (UMII) for his assistance in generating the OTU table for analysis and Aaron Rhendal for his assistance in statistical analysis of shedding data. For help in collection of samples and technical assistance we wish to thank: Fabian Chamba, Talita Resende, Yonghyan Kim, Luiza Roos, Jorge Garrido, Britta Wass, Kyrstin Danielson, Grace Moores and Jackelyn Goldschmidt. We would like to thank Dana Beckler for preparation of the Lawsonia intracellularis culture and assistance in challenge of animals. We would also like to thank the University of Minnesota Genomics Center for sequencing of samples for microbiome analysis. This work was supported by USDA-AFRI grant 1005423. Publisher Copyright: © 2018 The Author(s).

PY - 2018/12/1

Y1 - 2018/12/1

N2 - Salmonella enterica serovar Typhimurium continues to be a major cause of foodborne illness worldwide and pork can serve as a source of infection. Co-infection of S. enterica with Lawsonia intracellularis, a common intestinal pathogen of swine, has been found as risk factor for increased S. enterica shedding. The objective of this study was to investigate if vaccination against L. intracellularis could lead to decreased S. Typhimurium shedding. To test this hypothesis, pigs were challenged with either S. Typhimurium or S. Typhimurium and L. intracellularis, with and without L. intracellularis vaccination (n = 9 per group). A non-challenged group served as a negative control. Vaccination decreased the shedding of S. Typhimurium in co-infected animals by 2.12 log10 organisms per gram of feces at 7 days post infection. Analysis of the microbiome showed that vaccination led to changes in the abundance of Clostridium species, including Clostridium butyricum, in addition to other compositional changes that may explain the protection mediated against S. Typhimurium. These results indicate that vaccination against L. intracellularis in co-infected herds may provide a new tool to increase food safety by helping to prevent S. enterica without the need for antibiotics.

AB - Salmonella enterica serovar Typhimurium continues to be a major cause of foodborne illness worldwide and pork can serve as a source of infection. Co-infection of S. enterica with Lawsonia intracellularis, a common intestinal pathogen of swine, has been found as risk factor for increased S. enterica shedding. The objective of this study was to investigate if vaccination against L. intracellularis could lead to decreased S. Typhimurium shedding. To test this hypothesis, pigs were challenged with either S. Typhimurium or S. Typhimurium and L. intracellularis, with and without L. intracellularis vaccination (n = 9 per group). A non-challenged group served as a negative control. Vaccination decreased the shedding of S. Typhimurium in co-infected animals by 2.12 log10 organisms per gram of feces at 7 days post infection. Analysis of the microbiome showed that vaccination led to changes in the abundance of Clostridium species, including Clostridium butyricum, in addition to other compositional changes that may explain the protection mediated against S. Typhimurium. These results indicate that vaccination against L. intracellularis in co-infected herds may provide a new tool to increase food safety by helping to prevent S. enterica without the need for antibiotics.

UR - http://www.scopus.com/inward/record.url?scp=85041959745&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85041959745&partnerID=8YFLogxK

U2 - 10.1038/s41598-018-21255-7

DO - 10.1038/s41598-018-21255-7

M3 - Article

C2 - 29434295

AN - SCOPUS:85041959745

SN - 2045-2322

VL - 8

JO - Scientific reports

JF - Scientific reports

IS - 1

M1 - 2857

ER -

Vaccination Against Lawsonia intracellularis Decreases Shedding of Salmonella enterica serovar Typhimurium in Co-Infected Pigs and Alters the Gut Microbiome

Abstract

Bibliographical note

Access

OpenUrl availability

Other files and links

Fingerprint

Cite this