Validation of Fusarium head blight resistance QTL in US winter wheat

Stine Petersen, Jeanette H. Lyerly, Anne L. McKendry, M. Sariful Islam, Gina Brown-Guedira, Christina Cowger, Yanhong Dong, J. Paul Murphy

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

Fusarium head blight (FHB), primarily caused by Fusarium graminearum Schwabe [telemorph: Gibberella zeae Schw. (Petch)], can significantly reduce the grain quality of wheat (Triticum aestivum L.) due to mycotoxin contamination. Two US soft red winter wheat cultivars, Bess and NC-Neuse, have moderate resistance to FHB. The objective of this study was to validate genomic regions associated with FHB resistance identified in previous studies involving NC-Neuse and the cultivar Truman, a full-sib of Bess. A total of 98 doubled haploid lines derived from the cross Bess ´ NC-Neuse were evaluated in inoculated, mist-irrigated field nurseries. The lines were evaluated for FHB incidence, severity, Fusarium-damaged kernels, and deoxynivalenol content in seven environments between 2011 and 2014. A 3338-cM linkage map was developed based on 4014 simple sequence repeat and single nucleotide polymorphism markers. Twelve quantitative trait loci (QTL) associated with FHB resistance were identified. NC-Neuse alleles provided resistance at QTL on five chromosomes and Bess alleles provided resistance at QTL on five other chromosomes. Alignment of linkage maps revealed that five of these QTL were overlapping with previously identified regions. Quantitative trait loci on chromosomes 1A, 4A, and 6A identified in this study overlapped with QTL regions identified in NC-Neuse, and QTL identified on chromosomes 2B and 3B overlapped with QTL regions identified in Truman. A preliminary test using Kompetitive Allele-Specific polymerase chain reaction assays on recent Uniform Southern Winter Wheat Scab Nursery entries showed that the assays developed for Qfhb.nc-2B.1 may be good candidates for use in marker-assisted selection.

Original languageEnglish (US)
Pages (from-to)1-12
Number of pages12
JournalCrop Science
Volume57
Issue number1
DOIs
StatePublished - Jan 1 2017

Bibliographical note

Funding Information:
This material is based on work supported by the USDA, under Agreement No. 58-6645-3-030. This is a cooperative project with the US Wheat & Barley Scab Initiative. Any opinions, findings, conclusions, or recommendations expressed in this publication are those of the authors and do not necessarily reflect the view of the USDA. The work was also supported by the North Carolina Small Grain Growers Association. We thank Rene Navarro and Julie Solomon for technical assistance in inoculum preparation as well as evaluation of the population in field nurseries. Dr. Consuelo Arellano from the Statistics Dep. and Dr. Thomas Isleib from the Dep. of Crop Science at North Carolina State Univ. were very kind in assisting with statistical analyses. We acknowledge Dr. Margaret Worthington for her help reviewing this paper. Finally, we thank Jared Smith, Kim Howell, and Sharon Williamson from the USDA-ARS Plant Science Research Unit in Raleigh, NC, for their technical assistance, and the USDA-ARS Cereal Crops Research Unit in Fargo, ND, where the SNP array genotyping was conducted.

Publisher Copyright:
© Crop Science Society of America.

Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.

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