TY - JOUR
T1 - Variability in testing for antineutrophil cytoplasmic antibodies
T2 - A survey of participants in the College of American Pathologists proficiency testing program
AU - Karlon, William J.
AU - Naides, Stanley J.
AU - Crosson, John T.
AU - Ansari, Mohammad Qasim
N1 - Publisher Copyright:
© 2016, College of American Pathologists. All rights reserved.
PY - 2016/6
Y1 - 2016/6
N2 - Context. - Variability in testing for antineutrophil cytoplasmic antibodies (ANCAs) contributes to confusion and controversy related to testing for vasculitis and other ANCA-associated diseases. Objectives. - To survey laboratory testing practices regarding ANCA testing and to investigate differences in testing algorithms. Design. - Supplemental questions were sent to the 333 laboratories participating in the College of American Pathologists proficiency testing program for ANCA as part of the Special Immunology S2 Survey. Results. - A total of 315 laboratories submitted responses to the supplemental questions. Only 88 of 315 participants (28%) reported using a combination of indirect immunofluorescence (IFA) and enzyme immunoassay (EIA) techniques as recommended by current guidelines, with a few additional labs using IFA and multiplex bead assay as an acceptable alternative to EIA. Other labs reported using only IFA, EIA, or multiplex bead assays. Conclusions. - A wide variety of testing algorithms are in use for ANCA testing despite evidence to suggest that a combination of IFA and EIA testing provides the most comprehensive information. Laboratories should inform clinicians clearly about testing practices and utility of testing in specific disease states.
AB - Context. - Variability in testing for antineutrophil cytoplasmic antibodies (ANCAs) contributes to confusion and controversy related to testing for vasculitis and other ANCA-associated diseases. Objectives. - To survey laboratory testing practices regarding ANCA testing and to investigate differences in testing algorithms. Design. - Supplemental questions were sent to the 333 laboratories participating in the College of American Pathologists proficiency testing program for ANCA as part of the Special Immunology S2 Survey. Results. - A total of 315 laboratories submitted responses to the supplemental questions. Only 88 of 315 participants (28%) reported using a combination of indirect immunofluorescence (IFA) and enzyme immunoassay (EIA) techniques as recommended by current guidelines, with a few additional labs using IFA and multiplex bead assay as an acceptable alternative to EIA. Other labs reported using only IFA, EIA, or multiplex bead assays. Conclusions. - A wide variety of testing algorithms are in use for ANCA testing despite evidence to suggest that a combination of IFA and EIA testing provides the most comprehensive information. Laboratories should inform clinicians clearly about testing practices and utility of testing in specific disease states.
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U2 - 10.5858/arpa.2015-0221-CP
DO - 10.5858/arpa.2015-0221-CP
M3 - Article
C2 - 27232346
AN - SCOPUS:84971450125
SN - 0003-9985
VL - 140
SP - 524
EP - 528
JO - Archives of Pathology and Laboratory Medicine
JF - Archives of Pathology and Laboratory Medicine
IS - 6
ER -