Microinjection of arginine vasopressin into the lateral septum and bed nucleus of the stria terminalis of male hamsters stimulates intense flank marking and flank gland grooming, while microinjections of vasopressin in sites immediately adjacent to these areas or in the lateral ventricle are ineffective. Microinjections of oxytocin, angiotensin II and the behaviorally active C-terminal fragment of vasopressin, metabolite neuropeptide, by comparison, do not stimulate flank marking. Effective sites for vasopressin injection are clearly superimposable upon autoradiographically defined sites of high V1-receptor density. Furthermore, vasopressin-sensitive neurons in the lateral septum and bed nucleus of the stria terminalis are necessary for the expression of naturally elicited flank marking since the microinjection of a V1-receptor antagonist into these sites was able to temporarily block flank marking triggered by odors from conspecifics.
Bibliographical noteFunding Information:
This work was supported by NIH grant NS23557 (C.F.F.), NS20311 and the Veterans Administration (D.M.D.), BRSG grant E851U9 from the Research Foundation for Mental Hygiene (M.P.) and NIH National Research Service Award 1 F32 A605456-01 from the National Institute on Aging (P.S.). We thank Judith Allardice for her secretarial assistance.
- Flank marking
- Vasopressin receptor antagonist