Verapamil abolished the enhancement of protein phosphorylation of brainstem mitochondria and synaptosomes from the hens dosed with tri-o-cresyl phosphate

Yi Jun Wu, Ming Li, Yu Xia Li, Wei Li, Jia Yin Dai, Xin Fu Leng

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10 Scopus citations

Abstract

To explore the changes of the endogenous phosphorylation of brainstem mitochondrial and synaptosomal proteins in adult hens dosed with tri-o-cresyl phosphate (TOCP) following the development of organophosphate-induced delayed neurotoxicity (OPIDN). Verapamil (7 mg/(kg day), i.m.) was given for 4 days. A dose of TOCP (750 mg/kg, p.o.) was administrated in second day after verapamil. Phosphorylation of the proteins from brainstem mitochondria and synaptosomes was assayed in vitro by using [γ-32P]ATP as phosphate donor. Radiolabeled proteins were separated by SDS-PAGE and visualized by autoradiography. The results showed that TOCP administration enhanced the phosphorylation of the cell organelle proteins (mitochondria: 60, 55, 45, and 20 kDa; synaptosomes: 65, 60, and 20 kDa), while verapamil abolished the enhancement induced by TOCP. Additionally, the reaction for the phosphorylation is catalyzed by the calcium/calmodulin protein kinase. Therefore, TOCP can enhance the phosphorylation of the brainstem mitochondrial and synaptosomal proteins from the hens with OPIDN; however, protection from the enhancement of the phosphorylation should be involved in the mechanisms of the amelioration of TOCP-induced delayed neurotoxicity by verapamil.

Original languageEnglish (US)
Pages (from-to)67-71
Number of pages5
JournalEnvironmental Toxicology and Pharmacology
Volume24
Issue number1
DOIs
StatePublished - Jul 2007
Externally publishedYes

Bibliographical note

Funding Information:
This work was supported by grants from the Innovation Program of Chinese Academy of Sciences (KSCX2-SW-128) and the National Key Technologies R&D Program (2006BAK02A02) and 863 Program (No. 2006AA06Z423). The authors would like to thank Professor Bai Juan for providing us with calmodulin.

Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.

Keywords

  • Hen
  • Mitochondrion
  • Protein phosphorylation
  • Synaptosome
  • Tri-o-cresyl phosphate
  • Verapamil

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