Virus-induced gene silencing using a modified betasatellite: A potential candidate for functional genomics of crops

Jitendra Kumar; Samatha Gunapati; Jitesh Kumar; Anita Kumari; Abhinav Kumar; Rakesh Tuli; Sudhir P. Singh

doi:10.1007/s00705-014-2039-x

Virus-induced gene silencing using a modified betasatellite: A potential candidate for functional genomics of crops

Jitendra Kumar, Samatha Gunapati, Jitesh Kumar, Anita Kumari, Abhinav Kumar, Rakesh Tuli, Sudhir P. Singh

Plant Pathology

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

Betasatellites are geminivirus-associated single-stranded DNA molecules that play an important role in symptom modulation. A VIGS vector was developed by modifying cotton leaf curl Multan betasatellite (CLCuMB). CLCuMB DNA was modified by replacing the βC1 gene with a multiple cloning site. The silencing ability of the modified CLCuMB was investigated by cloning a fragment of a host gene (Su) or a reporter transgene (uidA) into the modified CLCuMB and co-agroinoculation with cotton leaf curl Multan virus, cotton leaf curl Kokhran virus, and ageratum enation virus, separately. The inoculated Nicotiana tabacum, N. benthamiana, Solanum lycopersicum, Arabidopsis thaliana and Gossypium hirsutum plants showed efficient silencing of the cognate genes.

Original language	English (US)
Pages (from-to)	2109-2113
Number of pages	5
Journal	Archives of Virology
Volume	159
Issue number	8
DOIs	https://doi.org/10.1007/s00705-014-2039-x
State	Published - Aug 2014

Bibliographical note

Funding Information:
This work was supported by Science and Engineering Research Board (SERB), Department of Science and Technology (DST), Government of India. SPS gratefully acknowledges the SERB grant (SR/FT/LS-88/2011). JK, SG and JK are thankful to CSIR, and AK to UGC (SAP), for providing fellowships.

Keywords

CLCuMB
PTGS
Trans-replication
VIGS

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abstract = "Betasatellites are geminivirus-associated single-stranded DNA molecules that play an important role in symptom modulation. A VIGS vector was developed by modifying cotton leaf curl Multan betasatellite (CLCuMB). CLCuMB DNA was modified by replacing the βC1 gene with a multiple cloning site. The silencing ability of the modified CLCuMB was investigated by cloning a fragment of a host gene (Su) or a reporter transgene (uidA) into the modified CLCuMB and co-agroinoculation with cotton leaf curl Multan virus, cotton leaf curl Kokhran virus, and ageratum enation virus, separately. The inoculated Nicotiana tabacum, N. benthamiana, Solanum lycopersicum, Arabidopsis thaliana and Gossypium hirsutum plants showed efficient silencing of the cognate genes.",

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AU - Kumar, Abhinav

AU - Tuli, Rakesh

AU - Singh, Sudhir P.

N1 - Funding Information: This work was supported by Science and Engineering Research Board (SERB), Department of Science and Technology (DST), Government of India. SPS gratefully acknowledges the SERB grant (SR/FT/LS-88/2011). JK, SG and JK are thankful to CSIR, and AK to UGC (SAP), for providing fellowships.

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Virus-induced gene silencing using a modified betasatellite: A potential candidate for functional genomics of crops

Abstract

Bibliographical note

Keywords

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