Virus production in microsphere-induced aggregate culture of animal cells

Cheryl M. Perusich; Stephane Goetghebeur; Wei Shou Hu

doi:10.1007/BF00159987

Virus production in microsphere-induced aggregate culture of animal cells

Cheryl M. Perusich, Stephane Goetghebeur, Wei Shou Hu

Chemical Engineering and Materials Science

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8 Scopus citations

Abstract

Green monkey kidney cells (Vero) were cultivated on microspheres with a median diameter of 23 μm. Aggregates were formed two days after inoculation and eventually reached a few hundred micrometers in diameter. Cells in these aggregates remained viable throughout the cultivation period and were successfully used for the production of vesicular stomatitis virus (VSV).

Original language	English (US)
Pages (from-to)	145-148
Number of pages	4
Journal	Biotechnology Techniques
Volume	5
Issue number	2
DOIs	https://doi.org/10.1007/BF00159987
State	Published - Mar 1991

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title = "Virus production in microsphere-induced aggregate culture of animal cells",

abstract = "Green monkey kidney cells (Vero) were cultivated on microspheres with a median diameter of 23 μm. Aggregates were formed two days after inoculation and eventually reached a few hundred micrometers in diameter. Cells in these aggregates remained viable throughout the cultivation period and were successfully used for the production of vesicular stomatitis virus (VSV).",

author = "Perusich, {Cheryl M.} and Stephane Goetghebeur and Hu, {Wei Shou}",

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AU - Goetghebeur, Stephane

AU - Hu, Wei Shou

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N2 - Green monkey kidney cells (Vero) were cultivated on microspheres with a median diameter of 23 μm. Aggregates were formed two days after inoculation and eventually reached a few hundred micrometers in diameter. Cells in these aggregates remained viable throughout the cultivation period and were successfully used for the production of vesicular stomatitis virus (VSV).

AB - Green monkey kidney cells (Vero) were cultivated on microspheres with a median diameter of 23 μm. Aggregates were formed two days after inoculation and eventually reached a few hundred micrometers in diameter. Cells in these aggregates remained viable throughout the cultivation period and were successfully used for the production of vesicular stomatitis virus (VSV).

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Virus production in microsphere-induced aggregate culture of animal cells

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