TY - JOUR
T1 - Voltammetric measurement of electrically evoked dopamine levels in the striatum of the anesthetized Syrian hamster
AU - Greco, Phillip G.
AU - Meisel, Robert L.
AU - Heidenreich, Byron A.
AU - Garris, Paul A.
N1 - Funding Information:
This work supported by NSF DBI-0138011 (PAG) and NIH DA 13680 (RLM).
PY - 2006/4/15
Y1 - 2006/4/15
N2 - Microdialysis measurements in the Syrian hamster clearly demonstrate a role for accumbal dopamine (DA) in female sexual behavior. However, large probe size and slow sampling rate prevent associating specific behaviors with DA changes in subregions of the heterogeneous nucleus accumbens. Fast-scan cyclic voltammetry (FSCV) at a carbon-fiber microelectrode (CFM), which affords millisecond temporal resolution at a micron-sized probe, could address these important issues. Mostly used in other rodents, e.g. rats and mice, this technique has not been applied to hamsters. The goal of the present study was to establish the measurement of DA in the nucleus accumbens of the anesthetized male Syrian hamster using FSCV at a CFM. For comparison, DA was simultaneously measured in the caudate-putamen. Stimulation of the medial forebrain bundle was used to elicit DA. Electrically evoked DA levels in both striatal regions were sensitive to location of the stimulating electrode and CFM, stimulation frequency, inhibition of DA uptake by cocaine and DA autoreceptor blockade by raclopride. Regional differences were observed for DA release and uptake parameters, and the effects of cocaine. Taken together, these results establish the measurement of electrically evoked DA levels in the hamster striatum using FSCV at a CFM.
AB - Microdialysis measurements in the Syrian hamster clearly demonstrate a role for accumbal dopamine (DA) in female sexual behavior. However, large probe size and slow sampling rate prevent associating specific behaviors with DA changes in subregions of the heterogeneous nucleus accumbens. Fast-scan cyclic voltammetry (FSCV) at a carbon-fiber microelectrode (CFM), which affords millisecond temporal resolution at a micron-sized probe, could address these important issues. Mostly used in other rodents, e.g. rats and mice, this technique has not been applied to hamsters. The goal of the present study was to establish the measurement of DA in the nucleus accumbens of the anesthetized male Syrian hamster using FSCV at a CFM. For comparison, DA was simultaneously measured in the caudate-putamen. Stimulation of the medial forebrain bundle was used to elicit DA. Electrically evoked DA levels in both striatal regions were sensitive to location of the stimulating electrode and CFM, stimulation frequency, inhibition of DA uptake by cocaine and DA autoreceptor blockade by raclopride. Regional differences were observed for DA release and uptake parameters, and the effects of cocaine. Taken together, these results establish the measurement of electrically evoked DA levels in the hamster striatum using FSCV at a CFM.
KW - Caudate-putamen
KW - Dopamine
KW - Nucleus accumbens
KW - Syrian hamster
KW - Voltammetry
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U2 - 10.1016/j.jneumeth.2005.08.009
DO - 10.1016/j.jneumeth.2005.08.009
M3 - Article
C2 - 16176838
AN - SCOPUS:33644760384
SN - 0165-0270
VL - 152
SP - 55
EP - 64
JO - Journal of Neuroscience Methods
JF - Journal of Neuroscience Methods
IS - 1-2
ER -