Water transport and IIF parameters for a connective tissue equivalent

Saravana Kumar Balasubramanian, John C Bischof, Allison Hubel

Research output: Contribution to journalArticlepeer-review

46 Scopus citations

Abstract

Understanding the biophysical processes that govern freezing injury of a tissue equivalent (TE) is an important step in characterizing and improving the cryopreservation of these systems. TEs were formed by entrapping human dermal fibroblasts (HDFs) in collagen or in fibrin gels. Freezing studies were conducted using a Linkam cryostage fitted to an optical microscope allowing observation of the TEs cooled under controlled rates between 5 and 130°C/min. Typically, freezing of cellular systems results in two biophysical processes that are both dependent on the cooling rate: dehydration and/or intracellular ice formation (IIF). Both these processes can potentially be destructive to cells. In this study, the biophysics of freezing cells in collagen and fibrin TEs have been quantified and compared to freezing cells in suspension. Experimental data were fitted in numerical models to extract parameters that governed water permeability, ELp and Lpg, and intracellular ice nucleation, Ωo and κo. Results indicate that major differences exist between freezing HDFs in suspension and in a tissue equivalent. During freezing, 55% of the HDFs in suspension formed IIF as compared to 100% of HDFs forming IIF in collagen and fibrin TE at a cooling rate of 130°C/min. Also, both the water permeability and the IIF parameters were determined to be higher for HDFs in TEs as compared to cell suspensions. Between the TEs, HDFs in fibrin TE exhibited higher values for the biophysical parameters as compared to HDFs in collagen TE. The observed biophysics seems to indicate that cell-cell and cell-matrix interactions play a major role in ice propagation in TEs.

Original languageEnglish (US)
Pages (from-to)62-73
Number of pages12
JournalCryobiology
Volume52
Issue number1
DOIs
StatePublished - Feb 1 2006

Keywords

  • Biophysics
  • Cryopreservation
  • Human dermal fibroblasts
  • Tissue equivalents

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