Widespread Rewiring of Genetic Networks upon Cancer Signaling Pathway Activation

Maximilian Billmann, Varun Chaudhary, Mostafa F. ElMaghraby, Bernd Fischer, Michael Boutros

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

Cellular signaling networks coordinate physiological processes in all multicellular organisms. Within networks, modules switch their function to control signaling activity in response to the cellular context. However, systematic approaches to map the interplay of such modules have been lacking. Here, we generated a context-dependent genetic interaction network of a metazoan's signaling pathway. Using Wnt signaling in Drosophila as a model, we measured >290,000 double perturbations of the pathway in a baseline state, after activation by Wnt ligand or after loss of the tumor suppressor APC. We found that genetic interactions within the Wnt network globally rewired after pathway activation. We derived between-state networks that showed how genes changed their function between state-specific networks. This related pathway inhibitors across states and identified genes required for pathway activation. For instance, we predicted and confirmed the ER-resident protein Catsup to be required for ligand-mediated Wnt signaling activation. Together, state-dependent and between-state genetic interaction networks identify responsive functional modules that control cellular pathways. Systematic measurement of genetic interactions between components of the Wnt pathway before and after pathway activation reveals how the pathway is rewired upon perturbation.

Original languageEnglish (US)
Pages (from-to)52-64.e4
JournalCell Systems
Volume6
Issue number1
DOIs
StatePublished - Jan 24 2018

Bibliographical note

Funding Information:
We would like to dedicate this paper to Bernd Fischer (deceased February 22, 2017). We thank Thomas Horn, Thomas Sandmann, Marija Buljan, Marco Breinig, and Chad Myers for helpful comments on the manuscript, and members of the Boutros lab for critical discussions. M. Billmann was in part supported by the Helmholtz International School of Cancer Research and an EMBO Short-term Fellowship ( ASTF 489-2014 ). B.F. was supported by the Helmholtz Association ( VH-NG-1010 ). Work in the lab of M. Boutros was funded by an ERC Advanced grant (“Syngene”) of the European Research Council .

Publisher Copyright:
© 2017 The Authors

Keywords

  • APC
  • Wnt
  • epistatic mapping
  • genetic interactions
  • signaling

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