WIN55,212-2-mediated inhibition of HIV-1 expression in microglial cells: Involvement of cannabinoid receptors

R B Rock; Genya Gekker; Shuxian Hu; Wen Sheng; Guy A. Cabral; Billy R. Martin; Phillip K. Peterson

doi:10.1007/s11481-006-9040-4

WIN55,212-2-mediated inhibition of HIV-1 expression in microglial cells: Involvement of cannabinoid receptors

R B Rock, Genya Gekker, Shuxian Hu, Wen Sheng, Guy A. Cabral, Billy R. Martin, Phillip K. Peterson

Medicine - Infectious Disease and International

Research output: Contribution to journal › Article › peer-review

46 Scopus citations

Abstract

Cannabinoid receptors CB₁ and CB₂ are primarily expressed in cells of the nervous and immune systems, respectively. Recently, the synthetic CB₁/CB₂ agonist WIN55,212-2 was found to suppress replication of HIV-1 in microglial cell cultures. The present study was undertaken to test the hypothesis that WIN55,212-2's antiviral effect is mediated via CB₂ receptors. By reverse transcription-polymerase chain reaction, microglia were found to express both CB₁ and CB ₂ receptors. Using additional CB₁/CB₂ receptor agonists and selective antagonists, we found that CB₂ receptors are involved in WIN55,212-2's antiviral activity and surprisingly that the CB ₁ receptor-selective antagonist SR141716A behaved as an agonist in these brain macrophages.

Original language	English (US)
Pages (from-to)	178-183
Number of pages	6
Journal	Journal of Neuroimmune Pharmacology
Volume	2
Issue number	2
DOIs	https://doi.org/10.1007/s11481-006-9040-4
State	Published - Jun 2007

Bibliographical note

Funding Information:
Acknowledgements This work was supported by grants from National Institute on Drug Abuse, DA-04381 and DA-09789, and was supported in part by U.S. Public Health Service grant DA-04381.

Keywords

Cannabinoids
HIV-1
Microglia
SR141716A
WIN55,212-2

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title = "WIN55,212-2-mediated inhibition of HIV-1 expression in microglial cells: Involvement of cannabinoid receptors",

abstract = "Cannabinoid receptors CB1 and CB2 are primarily expressed in cells of the nervous and immune systems, respectively. Recently, the synthetic CB1/CB2 agonist WIN55,212-2 was found to suppress replication of HIV-1 in microglial cell cultures. The present study was undertaken to test the hypothesis that WIN55,212-2's antiviral effect is mediated via CB2 receptors. By reverse transcription-polymerase chain reaction, microglia were found to express both CB1 and CB 2 receptors. Using additional CB1/CB2 receptor agonists and selective antagonists, we found that CB2 receptors are involved in WIN55,212-2's antiviral activity and surprisingly that the CB 1 receptor-selective antagonist SR141716A behaved as an agonist in these brain macrophages.",

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AU - Sheng, Wen

AU - Cabral, Guy A.

AU - Martin, Billy R.

AU - Peterson, Phillip K.

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AB - Cannabinoid receptors CB1 and CB2 are primarily expressed in cells of the nervous and immune systems, respectively. Recently, the synthetic CB1/CB2 agonist WIN55,212-2 was found to suppress replication of HIV-1 in microglial cell cultures. The present study was undertaken to test the hypothesis that WIN55,212-2's antiviral effect is mediated via CB2 receptors. By reverse transcription-polymerase chain reaction, microglia were found to express both CB1 and CB 2 receptors. Using additional CB1/CB2 receptor agonists and selective antagonists, we found that CB2 receptors are involved in WIN55,212-2's antiviral activity and surprisingly that the CB 1 receptor-selective antagonist SR141716A behaved as an agonist in these brain macrophages.

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WIN55,212-2-mediated inhibition of HIV-1 expression in microglial cells: Involvement of cannabinoid receptors

Abstract

Bibliographical note

Keywords

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