2-Amino-3-methylimidazo [4,5-f]quinoline-mediated immunosuppression involves inhibition of protein kinase C in murine splenocytes

Yong Woo Lee; Young Jin Jeon; Byung Sun Yoo; Kyu Hwan Yang

doi:10.1016/0304-3835(96)04356-X

2-Amino-3-methylimidazo [4,5-f]quinoline-mediated immunosuppression involves inhibition of protein kinase C in murine splenocytes

Yong Woo Lee, Young Jin Jeon, Byung Sun Yoo, Kyu Hwan Yang

Hormel Institute

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

The effects of the food-borne mutagenic and carcinogenic heterocyclic amine, 2-amino-3-methylimidazo[4,5-f]quinoline (IQ), on the proliferation of murine spleen cells was examined. A significant and dose-related suppression of the proliferative response to concanavalin A (Con A) was observed by the treatment of IQ. IQ also decreased markedly the level of proliferative response induced by PMA and ionomycin and the IL-2 secretion by T cells. Treatment of PMA resulted in the recovery of IQ-induced suppression of IL-2 production, but the addition of ionomycin (Io) had no effect. IQ decreased PKC activity in both the membrane fraction and the cytosol fraction and inhibited the phosphorylation of MARCKS protein. These results suggest that the suppression of spleen cell proliferation induced by IQ might be associated with the inhibition of PKC activity and the subsequent IL-2 secretion of T cells.

Original language	English (US)
Pages (from-to)	187-191
Number of pages	5
Journal	Cancer Letters
Volume	107
Issue number	2
DOIs	https://doi.org/10.1016/0304-3835(96)04356-X
State	Published - Oct 22 1996

Bibliographical note

Funding Information:
This work was supported by the Ministry Science and Technology grant, ROK.

Keywords

2-Amino-3-methylimidazo[4,5-f]quinoline (IQ)
Immunosuppression
Interleukin-2
MARCKS
Protein kinase C

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title = "2-Amino-3-methylimidazo [4,5-f]quinoline-mediated immunosuppression involves inhibition of protein kinase C in murine splenocytes",

abstract = "The effects of the food-borne mutagenic and carcinogenic heterocyclic amine, 2-amino-3-methylimidazo[4,5-f]quinoline (IQ), on the proliferation of murine spleen cells was examined. A significant and dose-related suppression of the proliferative response to concanavalin A (Con A) was observed by the treatment of IQ. IQ also decreased markedly the level of proliferative response induced by PMA and ionomycin and the IL-2 secretion by T cells. Treatment of PMA resulted in the recovery of IQ-induced suppression of IL-2 production, but the addition of ionomycin (Io) had no effect. IQ decreased PKC activity in both the membrane fraction and the cytosol fraction and inhibited the phosphorylation of MARCKS protein. These results suggest that the suppression of spleen cell proliferation induced by IQ might be associated with the inhibition of PKC activity and the subsequent IL-2 secretion of T cells.",

keywords = "2-Amino-3-methylimidazo[4,5-f]quinoline (IQ), Immunosuppression, Interleukin-2, MARCKS, Protein kinase C",

author = "Lee, {Yong Woo} and Jeon, {Young Jin} and Yoo, {Byung Sun} and Yang, {Kyu Hwan}",

note = "Funding Information: This work was supported by the Ministry Science and Technology grant, ROK.",

year = "1996",

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doi = "10.1016/0304-3835(96)04356-X",

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AU - Lee, Yong Woo

AU - Jeon, Young Jin

AU - Yoo, Byung Sun

AU - Yang, Kyu Hwan

N1 - Funding Information: This work was supported by the Ministry Science and Technology grant, ROK.

PY - 1996/10/22

Y1 - 1996/10/22

N2 - The effects of the food-borne mutagenic and carcinogenic heterocyclic amine, 2-amino-3-methylimidazo[4,5-f]quinoline (IQ), on the proliferation of murine spleen cells was examined. A significant and dose-related suppression of the proliferative response to concanavalin A (Con A) was observed by the treatment of IQ. IQ also decreased markedly the level of proliferative response induced by PMA and ionomycin and the IL-2 secretion by T cells. Treatment of PMA resulted in the recovery of IQ-induced suppression of IL-2 production, but the addition of ionomycin (Io) had no effect. IQ decreased PKC activity in both the membrane fraction and the cytosol fraction and inhibited the phosphorylation of MARCKS protein. These results suggest that the suppression of spleen cell proliferation induced by IQ might be associated with the inhibition of PKC activity and the subsequent IL-2 secretion of T cells.

AB - The effects of the food-borne mutagenic and carcinogenic heterocyclic amine, 2-amino-3-methylimidazo[4,5-f]quinoline (IQ), on the proliferation of murine spleen cells was examined. A significant and dose-related suppression of the proliferative response to concanavalin A (Con A) was observed by the treatment of IQ. IQ also decreased markedly the level of proliferative response induced by PMA and ionomycin and the IL-2 secretion by T cells. Treatment of PMA resulted in the recovery of IQ-induced suppression of IL-2 production, but the addition of ionomycin (Io) had no effect. IQ decreased PKC activity in both the membrane fraction and the cytosol fraction and inhibited the phosphorylation of MARCKS protein. These results suggest that the suppression of spleen cell proliferation induced by IQ might be associated with the inhibition of PKC activity and the subsequent IL-2 secretion of T cells.

KW - 2-Amino-3-methylimidazo[4,5-f]quinoline (IQ)

KW - Immunosuppression

KW - Interleukin-2

KW - MARCKS

KW - Protein kinase C

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2-Amino-3-methylimidazo [4,5-f]quinoline-mediated immunosuppression involves inhibition of protein kinase C in murine splenocytes

Abstract

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