A conserved RNA polymerase III promoter required for gammaherpesvirus TMER transcription and microRNA processing

Kevin W. Diebel; David J. Claypool; Linda F. van Dyk

doi:10.1016/j.gene.2014.04.026

A conserved RNA polymerase III promoter required for gammaherpesvirus TMER transcription and microRNA processing

Kevin W. Diebel, David J. Claypool, Linda F. van Dyk

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23 Scopus citations

Abstract

Canonical RNA polymerase III (pol III) type 2 promoters contain a single A and B box and are well documented for their role in tRNA and SINE transcription in eukaryotic cells. The genome of Murid herpesvirus 4 (MuHV-4) contains eight polycistronic tRNA-microRNA encoded RNA (TMER) genes that are transcribed from a RNA pol III type 2-like promoter containing triplicated A box elements. Here, we demonstrate that the triplicated A box sequences are required in their entirety to produce functional MuHV-4 miRNAs. We also identify that these RNA pol III type 2-like promoters are conserved in eukaryotic genomes. Human and mouse predicted tRNA genes containing these promoters also show enrichment of alternative RNA pol III transcription termination sequences and are predicted to give rise to longer tRNA primary transcripts.

Original language	English (US)
Pages (from-to)	8-18
Number of pages	11
Journal	Gene
Volume	544
Issue number	1
DOIs	https://doi.org/10.1016/j.gene.2014.04.026
State	Published - Jul 1 2014

Bibliographical note

Funding Information:
This work was supported by the National Institutes of Health [ RO1CA103632 to L.F.v.D., 2T32NS7321-21A1 to K.W.D.]; the Colorado Center for AIDS Research [ P30 AI054907 to L.F.v.D.]; and the Burroughs Wellcome Fund Foundation Investigator in Infectious Disease Award [100553 to L.F.v.D]. Funding for open access charge: [National Institutes of Health/ R56AI091994 ].

Keywords

A box
Gammaherpesvirus 68
TRNA
Type 2 promoter

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abstract = "Canonical RNA polymerase III (pol III) type 2 promoters contain a single A and B box and are well documented for their role in tRNA and SINE transcription in eukaryotic cells. The genome of Murid herpesvirus 4 (MuHV-4) contains eight polycistronic tRNA-microRNA encoded RNA (TMER) genes that are transcribed from a RNA pol III type 2-like promoter containing triplicated A box elements. Here, we demonstrate that the triplicated A box sequences are required in their entirety to produce functional MuHV-4 miRNAs. We also identify that these RNA pol III type 2-like promoters are conserved in eukaryotic genomes. Human and mouse predicted tRNA genes containing these promoters also show enrichment of alternative RNA pol III transcription termination sequences and are predicted to give rise to longer tRNA primary transcripts.",

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note = "Funding Information: This work was supported by the National Institutes of Health [ RO1CA103632 to L.F.v.D., 2T32NS7321-21A1 to K.W.D.]; the Colorado Center for AIDS Research [ P30 AI054907 to L.F.v.D.]; and the Burroughs Wellcome Fund Foundation Investigator in Infectious Disease Award [100553 to L.F.v.D]. Funding for open access charge: [National Institutes of Health/ R56AI091994 ]. ",

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AU - van Dyk, Linda F.

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KW - A box

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A conserved RNA polymerase III promoter required for gammaherpesvirus TMER transcription and microRNA processing

Abstract

Bibliographical note

Keywords

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