A contradictory GLABRA3 allele helps define gene interactions controlling trichome development in Arabidopsis

Jeffrey J. Esch, Margaret Chen, Mark A Sanders, Matthew Hillestad, Sampson Ndkium, Brian Idelkope, James Neizer, M D Marks

Research output: Contribution to journalArticlepeer-review

146 Scopus citations

Abstract

Previously characterized Arabidopsis gl3 mutants have trichomes that are smaller, less branched and undergo fewer rounds of endoreplication than wild-type trichomes. A new gl3 mutant, called gl3-sst, as oddly shaped trichomes that over expand during early development, undergo more endoreduplication and that have a striking nuclear morphology. The mutant nuclei consist of many interconnected lobes; however, only a single set of polytene-like chromosomes reside in the mutant nuclei. The predicted gl3-sst polypeptide has a Len to Phe substitution (codon 78) within a region responsible for protein-protein interaction. Yeast interaction assays comparing GL3 with gl3-sst proteins show that the mutant protein interaction with GM and TTG1 is decreased by 75% and 50%, respectively, but there is no difference in its interaction with TRY. Furthermore, TRY has the ability to prevent the GL1 GL3 interaction and the GL1 gl3-sst interaction is even more sensitive to TRY. Analysis of plants expressing functional GFP-tagged versions of GM, GL3 and TRY show that the proteins are localized in trichome nuclei. These results have been used to model trichome initiation in terms of protein interactions and threshold levels of activator complex.

Original languageEnglish (US)
Pages (from-to)5885-5894
Number of pages10
JournalDevelopment
Volume130
Issue number24
DOIs
StatePublished - Dec 2003

Keywords

  • CPC
  • Cell cycle
  • Cell fate
  • Chromosomes
  • Cytoskeleton
  • Differentiation
  • Elemental analysis
  • Endoreduplication
  • Endoreplication
  • GFP
  • GL1
  • GL3
  • Interphase
  • Lac OP/I-GFP
  • MYB
  • Nuclear localization
  • Root hair
  • TRY
  • TTG1
  • Transcription factor
  • Trichome
  • WER
  • bHLH
  • pBridge

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