Abstract
We describe a method that can be used to obtain and sequence 3′ and 5′ ends of cDNA transcripts directly from PCR products. The method employs a modified oligo(dT) primer that enables it to "lock-dock" at the junction of gene-specific cDNA sequence and a natural (3′) or appended (5′) poly(A) tail. As a result, discrete, first-round PCR products are obtained that are easily isolated and sequenced directly.
Original language | English (US) |
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Pages (from-to) | 144-148 |
Number of pages | 5 |
Journal | Genome research |
Volume | 2 |
Issue number | 2 |
DOIs | |
State | Published - 1992 |