A nanoparticle dispersion method for in vitro and in vivo nanotoxicity study

Seong C. Kim; Da Ren Chen; Chaolong Qi; Robert M. Gelein; Jacob N. Finkelstein; Alison Elder; Karen Bentley; Günter Oberdörster; David Y.H. Pui

doi:10.3109/17435390903374019

A nanoparticle dispersion method for in vitro and in vivo nanotoxicity study

Seong C. Kim, Da Ren Chen, Chaolong Qi, Robert M. Gelein, Jacob N. Finkelstein, Alison Elder, Karen Bentley, Günter Oberdörster, David Y.H. Pui

Mechanical Engineering

Research output: Contribution to journal › Article › peer-review

57 Scopus citations

Abstract

The dispersion in air of nanoparticles of different sizes, materials and morphologies with controlled agglomeration involving aerosol delivery for in vivo and in vitro studies is one of the most difficult challenges in the field of nanoparticle toxicology. We describe here a nanoparticle dispersion system using an electrospray method to deliver airborne nanoparticles (∼ 10-100 nm) with spatial uniformity and controllable particle concentration for in vitro and in vivo studies. With the dispersion method, single nanoparticles (polystyrene latex particles, TiO₂, Au, Mn, quantum dots, and carbon nanotubes) can be delivered to cells and animals via the air. The degree of agglomeration can be controlled by changing the suspension feeding rate to simulate realistic conditions for exposure studies.

Original language	English (US)
Pages (from-to)	42-51
Number of pages	10
Journal	Nanotoxicology
Volume	4
Issue number	1
DOIs	https://doi.org/10.3109/17435390903374019
State	Published - Mar 2010

Bibliographical note

Funding Information:
Declaration of interest: This work was supported by a grant from the U.S. Department of Defense (AFOSR) MURI Grant (FA9550-04-1-0430). Parts of this work were carried out in the Minnesota Characterization Facility and the Particle Technology Laboratory, which receive partial support from NSF through the NNIN program.

Keywords

Aerosols
Electrospray
In vitro
In vivo
Nanoparticles
Toxicology

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abstract = "The dispersion in air of nanoparticles of different sizes, materials and morphologies with controlled agglomeration involving aerosol delivery for in vivo and in vitro studies is one of the most difficult challenges in the field of nanoparticle toxicology. We describe here a nanoparticle dispersion system using an electrospray method to deliver airborne nanoparticles (∼ 10-100 nm) with spatial uniformity and controllable particle concentration for in vitro and in vivo studies. With the dispersion method, single nanoparticles (polystyrene latex particles, TiO2, Au, Mn, quantum dots, and carbon nanotubes) can be delivered to cells and animals via the air. The degree of agglomeration can be controlled by changing the suspension feeding rate to simulate realistic conditions for exposure studies.",

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note = "Funding Information: Declaration of interest: This work was supported by a grant from the U.S. Department of Defense (AFOSR) MURI Grant (FA9550-04-1-0430). Parts of this work were carried out in the Minnesota Characterization Facility and the Particle Technology Laboratory, which receive partial support from NSF through the NNIN program.",

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AU - Kim, Seong C.

AU - Chen, Da Ren

AU - Qi, Chaolong

AU - Gelein, Robert M.

AU - Finkelstein, Jacob N.

AU - Elder, Alison

AU - Bentley, Karen

AU - Oberdörster, Günter

AU - Pui, David Y.H.

N1 - Funding Information: Declaration of interest: This work was supported by a grant from the U.S. Department of Defense (AFOSR) MURI Grant (FA9550-04-1-0430). Parts of this work were carried out in the Minnesota Characterization Facility and the Particle Technology Laboratory, which receive partial support from NSF through the NNIN program.

PY - 2010/3

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N2 - The dispersion in air of nanoparticles of different sizes, materials and morphologies with controlled agglomeration involving aerosol delivery for in vivo and in vitro studies is one of the most difficult challenges in the field of nanoparticle toxicology. We describe here a nanoparticle dispersion system using an electrospray method to deliver airborne nanoparticles (∼ 10-100 nm) with spatial uniformity and controllable particle concentration for in vitro and in vivo studies. With the dispersion method, single nanoparticles (polystyrene latex particles, TiO2, Au, Mn, quantum dots, and carbon nanotubes) can be delivered to cells and animals via the air. The degree of agglomeration can be controlled by changing the suspension feeding rate to simulate realistic conditions for exposure studies.

AB - The dispersion in air of nanoparticles of different sizes, materials and morphologies with controlled agglomeration involving aerosol delivery for in vivo and in vitro studies is one of the most difficult challenges in the field of nanoparticle toxicology. We describe here a nanoparticle dispersion system using an electrospray method to deliver airborne nanoparticles (∼ 10-100 nm) with spatial uniformity and controllable particle concentration for in vitro and in vivo studies. With the dispersion method, single nanoparticles (polystyrene latex particles, TiO2, Au, Mn, quantum dots, and carbon nanotubes) can be delivered to cells and animals via the air. The degree of agglomeration can be controlled by changing the suspension feeding rate to simulate realistic conditions for exposure studies.

KW - Aerosols

KW - Electrospray

KW - In vitro

KW - In vivo

KW - Nanoparticles

KW - Toxicology

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A nanoparticle dispersion method for in vitro and in vivo nanotoxicity study

Abstract

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Keywords

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