A new method for detecting nucleolus organizer regions in fish chromosomes using denaturation and propidium iodide staining

Petr Rab; Kent M. Reed; F. Abel Ponce De León; Ruth B. Phillips

doi:10.3109/10520299609117153

A new method for detecting nucleolus organizer regions in fish chromosomes using denaturation and propidium iodide staining

Petr Rab, Kent M. Reed, F. Abel Ponce De León, Ruth B. Phillips

Research output: Contribution to journal › Article › peer-review

41 Scopus citations

Abstract

A rapid method for detecting nucleolus organizer regions (NORs) in fish chromosomes based on thermal denaturation and staining with propidium iodide is described. Under epifluorescence, the NORs of 15 fish species from six families could be detected. This protocol differentiates constitutive heterochromatin in mammalian and avian chromosomes, and in some cases, heterochromatic blocks in fish chromosomes. The staining of NORs of fish chromosomes with propidium iodide following denaturation with formalin is likely the result of differential denaturation of the rDNA due to the thermal characteristics of AT- and GC-rich domains of the rDNA cistron. This technique provides a new useful marker for descriptive fish cytogenetic studies.

Original language	English (US)
Pages (from-to)	157-162
Number of pages	6
Journal	Biotechnic and Histochemistry
Volume	71
Issue number	3
DOIs	https://doi.org/10.3109/10520299609117153
State	Published - May 1996
Externally published	Yes

Bibliographical note

Funding Information:
This study was supported by grants from the US National Academy of Sciences (PR and RBP), the NRI Competitive Grants Program/USDA (93-37205-9171t o KMR). and the National Science Foundation (DEB 91 19293 to RBP).

Keywords

DPI staining
fish chromosomes
nucleolus organizer region
rDNA genes

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title = "A new method for detecting nucleolus organizer regions in fish chromosomes using denaturation and propidium iodide staining",

abstract = "A rapid method for detecting nucleolus organizer regions (NORs) in fish chromosomes based on thermal denaturation and staining with propidium iodide is described. Under epifluorescence, the NORs of 15 fish species from six families could be detected. This protocol differentiates constitutive heterochromatin in mammalian and avian chromosomes, and in some cases, heterochromatic blocks in fish chromosomes. The staining of NORs of fish chromosomes with propidium iodide following denaturation with formalin is likely the result of differential denaturation of the rDNA due to the thermal characteristics of AT- and GC-rich domains of the rDNA cistron. This technique provides a new useful marker for descriptive fish cytogenetic studies.",

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author = "Petr Rab and Reed, {Kent M.} and {Ponce De Le{\'o}n}, {F. Abel} and Phillips, {Ruth B.}",

note = "Funding Information: This study was supported by grants from the US National Academy of Sciences (PR and RBP), the NRI Competitive Grants Program/USDA (93-37205-9171t o KMR). and the National Science Foundation (DEB 91 19293 to RBP).",

year = "1996",

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doi = "10.3109/10520299609117153",

language = "English (US)",

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AU - Rab, Petr

AU - Reed, Kent M.

AU - Ponce De León, F. Abel

AU - Phillips, Ruth B.

N1 - Funding Information: This study was supported by grants from the US National Academy of Sciences (PR and RBP), the NRI Competitive Grants Program/USDA (93-37205-9171t o KMR). and the National Science Foundation (DEB 91 19293 to RBP).

PY - 1996/5

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N2 - A rapid method for detecting nucleolus organizer regions (NORs) in fish chromosomes based on thermal denaturation and staining with propidium iodide is described. Under epifluorescence, the NORs of 15 fish species from six families could be detected. This protocol differentiates constitutive heterochromatin in mammalian and avian chromosomes, and in some cases, heterochromatic blocks in fish chromosomes. The staining of NORs of fish chromosomes with propidium iodide following denaturation with formalin is likely the result of differential denaturation of the rDNA due to the thermal characteristics of AT- and GC-rich domains of the rDNA cistron. This technique provides a new useful marker for descriptive fish cytogenetic studies.

AB - A rapid method for detecting nucleolus organizer regions (NORs) in fish chromosomes based on thermal denaturation and staining with propidium iodide is described. Under epifluorescence, the NORs of 15 fish species from six families could be detected. This protocol differentiates constitutive heterochromatin in mammalian and avian chromosomes, and in some cases, heterochromatic blocks in fish chromosomes. The staining of NORs of fish chromosomes with propidium iodide following denaturation with formalin is likely the result of differential denaturation of the rDNA due to the thermal characteristics of AT- and GC-rich domains of the rDNA cistron. This technique provides a new useful marker for descriptive fish cytogenetic studies.

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KW - rDNA genes

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A new method for detecting nucleolus organizer regions in fish chromosomes using denaturation and propidium iodide staining

Abstract

Bibliographical note

Keywords

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