A pathway for biological methane production using bacterial iron-only nitrogenase

Yanning Zheng, Derek F. Harris, Zheng Yu, Yanfen Fu, Saroj Poudel, Rhesa N. Ledbetter, Kathryn R. Fixen, Zhi Yong Yang, Eric S. Boyd, Mary E. Lidstrom, Lance C. Seefeldt, Caroline S. Harwood

Research output: Contribution to journalArticlepeer-review

46 Scopus citations

Abstract

Methane (CH 4 ) is a potent greenhouse gas that is released from fossil fuels and is also produced by microbial activity, with at least one billion tonnes of CH 4 being formed and consumed by microorganisms in a single year 1 . Complex methanogenesis pathways used by archaea are the main route for bioconversion of carbon dioxide (CO 2 ) to CH 4 in nature 2-4 . Here, we report that wild-type iron-iron (Fe-only) nitrogenase from the bacterium Rhodopseudomonas palustris reduces CO 2 simultaneously with nitrogen gas (N 2 ) and protons to yield CH 4 , ammonia (NH 3 ) and hydrogen gas (H 2 ) in a single enzymatic step. The amount of CH 4 produced by purified Fe-only nitrogenase was low compared to its other products, but CH 4 production by this enzyme in R. palustris was sufficient to support the growth of an obligate CH 4 -utilizing Methylomonas strain when the two microorganisms were grown in co-culture, with oxygen (O 2 ) added at intervals. Other nitrogen-fixing bacteria that we tested also formed CH 4 when expressing Fe-only nitrogenase, suggesting that this is a general property of this enzyme. The genomes of 9% of diverse nitrogen-fixing microorganisms from a range of environments encode Fe-only nitrogenase. Our data suggest that active Fe-only nitrogenase, present in diverse microorganisms, contributes CH 4 that could shape microbial community interactions.

Original languageEnglish (US)
Pages (from-to)281-286
Number of pages6
JournalNature Microbiology
Volume3
Issue number3
DOIs
StatePublished - Mar 1 2018

Fingerprint Dive into the research topics of 'A pathway for biological methane production using bacterial iron-only nitrogenase'. Together they form a unique fingerprint.

Cite this