A practical and scalable manufacturing process for an antifungal agent, nikkomycin Z

Christopher J. Stenland; Lev G. Lis; Frederick J. Schendel; Nicholas J. Hahn; Mary A. Smart; Amy L. Miller; Marc G. Von Keitz; Vadim J. Gurvich

doi:10.1021/op3003294

A practical and scalable manufacturing process for an antifungal agent, nikkomycin Z

Christopher J. Stenland, Lev G. Lis, Frederick J. Schendel, Nicholas J. Hahn, Mary A. Smart, Amy L. Miller, Marc G. Von Keitz, Vadim J. Gurvich

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14 Scopus citations

Abstract

A scalable and reliable manufacturing process for nikkomycin Z HCl on a scale of 170 g has been developed and optimized. The process is characterized by a 2.3 g/L fermentation yield, 79% purification yield, and >98% relative purity of the final product. This method is suitable for further scale-up and cGMP production. The Streptomyces tendae ΔNikQ strain developed during the course of this study is superior to any previously reported strain in terms of higher yield and purity of nikkomycin Z.

Original language	English (US)
Pages (from-to)	265-272
Number of pages	8
Journal	Organic Process Research and Development
Volume	17
Issue number	2
DOIs	https://doi.org/10.1021/op3003294
State	Published - Feb 15 2013

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abstract = "A scalable and reliable manufacturing process for nikkomycin Z HCl on a scale of 170 g has been developed and optimized. The process is characterized by a 2.3 g/L fermentation yield, 79% purification yield, and >98% relative purity of the final product. This method is suitable for further scale-up and cGMP production. The Streptomyces tendae ΔNikQ strain developed during the course of this study is superior to any previously reported strain in terms of higher yield and purity of nikkomycin Z.",

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AU - Lis, Lev G.

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AU - Smart, Mary A.

AU - Miller, Amy L.

AU - Von Keitz, Marc G.

AU - Gurvich, Vadim J.

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A practical and scalable manufacturing process for an antifungal agent, nikkomycin Z

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